Utilize the in vitro 3D Non-alcoholic steatohepatitis (NASH), also known as Metabolic-dysfunction-associated steatohepatitis (MASH) model to expedite the path to effective treatment development.

Everything needed to replicate the industry-validated human in vitro NASH model in the user’s own laboratory is included in NASH-in-a-box.

It is intended to be used with the single- and multi-organ microphysiological systems in the PhysioMimix OOC range.

NASH-in-a-box supports:

  • The swift acceptance of an organ-on-a-chip methodology by scientists, regardless of their background
  • Instant access to protocols that have been verified and improved over years of industry cooperation
  • Producing clinically translatable data with high content to reveal disease mechanisms and validate medication safety and effectiveness

Features of the 3D NASH model kit

Use NASH-in-a-box to overcome the drawbacks of conventional methods and prevent unanticipated outcomes in the clinic.

  • 10-month shelf life (from manufacture)
  • ELISA quality control kits (albumin/cytotoxicity) to test microtissue health and function
  • PhysioMimix OOC compatible and fully supported
  • Guaranteed  for 14 days of culture
  • Proprietary NASH media and supplements
  • Software-guided protocols
  • 3D-validated primary human hepatocytes, stellate, and Kupffer cells

What is in the box?

Media. Source: CN-Bio

Media Volume
Hepatocyte thawing media 50 mL
Basal media (HEP-Fat) 500 mL
Basal media (HEP-Stellate) 250 mL
Basal media (HEP-Seeding) 250 mL

 

Supplements. Source: CN-Bio

Supplements Volume
HEP- Supplement A 5 x 2.5 mL
HEP Supplement B 1 x 250 µL  
HEP- Supplement C 1 x 500 µL  
HEP- Supplement D 1 x 500 µL  
Hydrocortisone 5 x 5 µL  
TGF – Beta 5 x 200 µL 

 

Assay kits. Source: CN-Bio

Quality control assays Number of kits
Assaypro Human Albumin AssayMax ELISA Kit 2 kits
Promega CytoTox 96 Cytotoxicity (LDH) Assay Kit 1 kit

 

Primary human cells. Source: CN-Bio

Primary human cells Number of cells  
Hepatocytes min 10M
Kupffer cells min 1M
Stellate cells min 100K

 

PhysioMimix plates. Source: CN-Bio

Multi-chip plates   Number of plates/replicates
Multi-chip Liver-12+ plate (MPS-LC12+) 2 plates /24 replicates  

 

Validation report

The lengthy procedure of verifying donor cell combinations within NASH-in-a-box is detailed in the sample report.

When cultured for 14 days, the quality control protocols guarantee the formation of stable, three-dimensional liver microtissues that exhibit a NASH phenotype.

PhysioMimix® Compatible NASH-in-a-Box

Image Credit: CN-Bio

Guided software

Guided software, which can be found on the PhysioMimix Controller, offers detailed instructions for culturing liver microtissues and causing the NASH phenotype.

PhysioMimix® Compatible NASH-in-a-Box

Image Credit: CN-Bio

It helps operators with little experience to confidently use the NASH-in-a-box kit by walking them through the process.

How does the 3D NASH model compare?

Preclinical research and development instruments are widely accessible. How does NASH-in-a-box compare to established approaches?

Source: CN-Bio

  In vitro 2D
cell culture
In vitro 3D
 
spheroid
In vivo 
animal models
NASH-in-a-
box
Human relevance ++ ++ + +++
Data content LOW MEDIUM HIGH HIGH
Longevity < 7 days < 7 days > 4 weeks 2 weeks
Model complexity + + +++ ++
Throughput +++ +++ + ++
Ease of adoption +++ ++ + ++
Cost $ $ $$$ $$
Time to result DAYS DAYS MONTHS WEEKS
NASH phenotype + ++ + +++

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