Chagas disease also called American trypanosomiasis is a tropical parasitic disease caused by the flagellate protozoan ''Trypanosoma cruzi''. ''T. cruzi'' is commonly transmitted to humans and other mammals by an insect vector, the blood-sucking assassin bugs of the subfamily Triatominae (family Reduviidae) most commonly species belonging to the ''Triatoma'', ''Rhodnius'', and ''Panstrongylus'' genera. The disease may also be spread through blood transfusion and organ transplantation, ingestion of food contaminated with parasites, and from a mother to her fetus.
The symptoms of Chagas disease vary over the course of an infection. In the early, acute stage, symptoms are mild and usually produce no more than local swelling at the site of infection. As the disease progresses, over the course of many years, serious chronic symptoms can appear, such as heart disease and malformation of the intestines. If untreated, the chronic disease is often fatal. Current drug treatments are generally unsatisfactory; available medications are highly toxic and often ineffective, particularly those used to treat the chronic stage of the disease.
Chagas disease occurs exclusively in the Americas, particularly in poor, rural areas of Mexico, Central America, and South America; very rarely, the disease has originated in the Southern United States. The insects that spread the disease are known by various local names, including ''vinchuca'' in Argentina, Bolivia and Paraguay, ''barbeiro'' (the barber) in Brazil, ''Pito'' in Colombia, ''chinche'' in Central America, ''chipo'', ''chupança'', ''chinchorro'', and "the kissing bug". It is estimated that as many as 8 to 11 million people in Mexico, Central America, and South America have Chagas disease, most of whom do not know they are infected. Large-scale population movements from rural to urban areas of Latin America and to other regions of the world have increased the geographic distribution of Chagas disease. Control strategies have mostly focused on eliminating the triatomine vector and preventing transmission from other sources. Although the symptoms resolve, the infection, if untreated, persists. Rarely, young children (<5%), or adults die from severe inflammation/infection of the heart muscle (myocarditis) or brain (meningoencephalitis). The acute phase also can be severe in people with weakened immune systems. and by accidental laboratory exposure. Chagas disease can also be spread congenitally (from a pregnant woman to her baby) through the placenta, and accounts for approximately 13% of stillborn deaths in parts of Brazil.
In 1991 farm workers in the state of Paraíba, Brazil, were infected by eating contaminated food; transmission has also occurred via contaminated açaí palm fruit juice and sugar cane juice. Despite many warnings in the press and by health authorities, this source of infection continues unabated.
Chagas Disease Diagnosis
The presence of ''T. cruzi'' is diagnostic of Chagas disease. It can be detected by Microscopic examination of fresh anti-coagulated blood, or its buffy coat, for motile parasites; or by preparation of thin and thick blood smears stained with Giemsa, for direct visualization of parasites. Microscopically, ''T. cruzi'' can be confused with ''Trypanosoma rangeli'', which is not known to be pathogenic in humans. Isolation of ''T. cruzi'' can occur by inoculation into mice, by culture in specialized media (e.g., NNN, LIT); and by xenodiagnosis, where uninfected Reduviidae bugs are fed on the patient's blood, and their gut contents examined for parasites. and prevention is generally focused on fighting the vector ''Triatoma'' by using sprays and paints containing insecticides (synthetic pyrethroids), and improving housing and sanitary conditions in rural areas. For urban dwellers, spending vacations and camping out in the wilderness or sleeping at hostels or mud houses in endemic areas can be dangerous; a mosquito net is recommended.
A number of potential vaccines are currently being tested. Vaccination with ''Trypanosoma rangeli'' has produced positive results in animal models. More recently, the potential of DNA vaccines for immunotherapy of acute and chronic Chagas disease is being tested by several research groups.
Blood transfusion is the second most common transmission route of Chagas disease in many Latin American countries. In 1993 a series of serologic surveys, looking for antibodies against ''T. cruzi'' in blood donors, revealed that the probability of receiving a potentially infected transfusion unit in each country varied from 1.4% to 18% in Argentina, Brazil, and Chile, and can be up to 48% in Bolivia. Suggesting that in this region there is a high risk of transfusion acquired Chagas; much higher than the risks reported for other infections acquired through blood such as hepatitis (0.1%) and AIDS (0.1%) in the same region. The likelihood of being infected when receiving an infected transfusion unit were assumed to be 20% for ''T. cruzi''. Vector control efforts have, however, led to a reduction in infection rates; the prevalence of ''T. cruzi'' infection in the Brazilian blood bank system was 0.96% in 1996, down from 2% in the 1970s. In most countries where Chagas disease is endemic, testing of blood donors is already mandatory, since this can be an important route of transmission. The United States FDA has recently licensed a test for antibodies against ''T. cruzi'' for use on blood donors but has not yet mandated its use. The American Association of Blood Banks recommends that past recipients of blood components from donors found to be infected be notified and themselves tested.
In the past, donated blood was mixed with 0.25 g/L of gentian violet, which kills ''T. cruzi'' parasites. The antifungal agent amphotericin B has been proposed as a second-line treatment, but the high cost and relatively high toxicity of the drug have limited its use.
Chagas Disease Management
There are two approaches to treating Chagas disease, antiparasitic treatment, to kill the parasite; and symptomatic treatment, to manage the symptoms and signs of infection.
Medication
Antiparasitic treatment is most effective early in the course of infection but is not limited to cases in the acute phase. Drugs of choice include azole or nitro derivatives such as benznidazole or nifurtimox. However, resistance to these drugs has been reported. Moreover, a 10-year study of chronic administration of antiparasitic drugs in Brazil has revealed that current drug treatment regimens do not fully remove parasitemia.
Management of complications
In the chronic stage, treatment involves managing the clinical manifestations of the disease. For example, pacemakers and medications for irregular heartbeats, such as the anti-arrhythmia drug amiodarone, may be life saving for some patients with chronic cardiac disease, while surgery may be required for megaintestine. The disease cannot be cured in this phase, however. Chronic heart disease caused by Chagas disease is now a common reason for heart transplantation surgery. Until recently, however, Chagas disease was considered a contraindication for the procedure, since the heart damage could recur as the parasite was expected to seize the opportunity provided by the immunosuppression that follows surgery. It was noted that survival rates in Chagas patients could be significantly improved by using lower dosages of the immunosuppressant drug ciclosporin. Recently, direct stem cell therapy of the heart muscle using bone marrow cell transplantation has been shown to dramatically reduce risks of heart failure in Chagas patients.
Chagas Disease Epidemiology
Chagas disease affects 16–18 million people as of 2008, with some 100 million (25% of the Latin American population) at risk of acquiring the disease, killing around 20,000 people annually. Chagas exists in two different ecological zones. In the Southern Cone region the main vector lives in and around human homes. In Central America and Mexico the main vector species lives both inside dwellings and in uninhabited areas. In both zones Chagas occurs almost exclusively in rural areas, where triatomine breed and feed on the over 150 species from 24 families of domestic and wild mammals, as well as humans, that are the natural reservoirs of ''T.cruzi''.
Dense vegetation (such as that of tropical rainforests) and urban habitats are not ideal for the establishment of the human transmission cycle. However, in regions where the sylvatic habitat and its fauna are thinned by economical exploitation and human habitation, such as in newly deforested areas, piassava palm culture areas, and some parts of the Amazon region, a human transmission cycle may develop as the insects search for new food sources.
The primary wildlife reservoirs for ''Trypanosoma cruzi'' in the United States include opossums, raccoons, armadillos, squirrels, woodrats and mice. Recorded prevalence of the disease in opossums in the U.S. ranges from 8.3% Studies on raccoons in the Southeast have yielded infection rates ranging from 47% to as low as 15.5%. Additionally small rodents including squirrels, mice and rats are important in the sylvatic transmission cycle because of their importance as bloodmeal sources for the insect vectors. A Texas study revealed 17.3% percent ''T. cruzi'' prevalence in 75 specimens comprised of four separate small rodent species.
Chronic Chagas disease remains a major health problem in many Latin American countries, despite the effectiveness of hygienic and preventive measures, such as eliminating the transmitting insects, which have reduced to zero new infections in at least two countries of the region. With increased population movements, however, the possibility of transmission by blood transfusion has become more substantial in the United States. Approximately 500,000 infected people live in the United States, which is likely the result of immigration from Latin American countries.
Several landmarks have been achieved in the fight against Chagas disease in Latin America including a reduction by 72% of the incidence of human infection in children and young adults in the countries of the Southern Cone Initiative, and at least two countries (Uruguay, in 1997, and Chile, in 1999) have been certified free of vectorial and transfusional transmission. In Argentina vectorial transmission has been interrupted in 13 of the 19 endemic provinces. although other vectors, particularly ''T. brasiliensis'' and ''T. pseudomaculata'', account for most transmission in the Northeast Region.
Some stepstones of vector control:
- A yeast trap has been tested for monitoring infestations of certain species of triatomine bugs (''Triatoma sordida'', ''Triatoma brasiliensis'', ''Triatoma pseudomaculata'', and ''Panstrongylus megistus'').
- Promising results were gained with the treatment of vector habitats with the fungus ''Beauveria bassiana''.
- Targeting the symbionts of Triatominae through paratransgenesis.
Chagas Disease History
The disease was named after the Brazilian physician and infectologist Carlos Chagas, who first described it in 1909, but the disease was not seen as a major public health problem in humans until the 1960s (the outbreak of Chagas disease in Brazil in the 1920s went widely ignored). He discovered that the intestines of Triatomidae (now Reduviidae: Triatominae) harbored a flagellate protozoan, a new species of the ''Trypanosoma'' genus, and was able to prove experimentally that it could be transmitted to marmoset monkeys that were bitten by the infected bug. Later studies showed that squirrel monkeys were also vulnerable to infection.
Chagas named the pathogenic parasite ''Trypanosoma cruzi'' both honoring Oswaldo Cruz, the noted Brazilian physician and epidemiologist who fought successfully epidemics of yellow fever,smallpox, and bubonic plague in Rio de Janeiro and other cities in the beginning of the 20th century. Chagas’ work is unique in the history of medicine because he was the only researcher so far to describe ''solely'' and completely a new infectious disease: itspathogen, vector, host, clinical manifestations, and epidemiology.
Nevertheless, he believed (falsely) until 1925 that the main infection route was by the bite of the insect—and not by its feces, as was proposed by his colleague Emile Brumpt in 1915 and assured by Silveira Dias in 1932, Cardoso in 1938, and Brumpt himself in 1939. Chagas was also the first to unknowingly discover and illustrate the parasitic fungal genus ''Pneumocystis'', later infamously to be linked to PCP (Pneumocystis pneumonia in AIDS victims)., in honor of Dr. Salvador Mazza, the Argentine doctor who in 1926 began investigating the disease and over the years became the principal researcher of this disease in the country. Dr. Mazza produced the first scientific confirmation in 1927 of the existence of ''trypanosoma cruzi'' in Argentina, eventually leading to support from local and European medical schools and Argentine government policy makers.
It has been hypothesized that Charles Darwin might have suffered from Chagas disease as a result of a bite of the so-called Great Black Bug of the Pampas (vinchuca) (see Charles Darwin's illness). The episode was reported by Darwin in his diaries of the Voyage of the Beagle as occurring in March 1835 to the east of the Andes near Mendoza. Darwin was young and generally in good health, though six months previously he had been ill for a month near Valparaiso, but in 1837, almost a year after he returned to England, he began to suffer intermittently from a strange group of symptoms, becoming incapacitated for much of the rest of his life. Attempts to test Darwin's remains at the Westminster Abbey by using modern PCR techniques were met with a refusal by the Abbey's curator.
Chagas Disease Research
Several experimental treatments have shown promise in animal models. These include inhibitors of oxidosqualene cyclase and squalene synthase, cysteine protease inhibitors, dermaseptins collected from frogs in the genus ''Phyllomedusa'' (''P. oreades'' and ''P. distincta''), the sesquiterpene lactone dehydroleucodine (DhL) which affects the growth of cultured epimastigotes of ''Trypanosoma cruzi'', inhibitors of purine uptake, It is hoped that new drug targets may be revealed following the sequencing of the ''T. cruzi'' genome.
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