Researchers have determined that a gene present in mouse cells limits the number of times that a cell can divide. The gene is involved in a process, called senescence, which is thought to ensure that aging cells do not pass on harmful mutations.
The researchers said the gene, known as SIRT1, suppresses longevity, and may play a role in regulating the aging process. But they caution against interpreting the results too broadly, because dividing mouse cells in culture are an imperfect model of how aging affects human cells.
There is, however, some indication from the new studies that suppressing SIRT1 could prove important in techniques that researchers use to generate large numbers of normal cells for research. In this context, the SIRT1-deficient cells hold an advantage over other highly proliferative cell types, such as cancer cells, because although they divide indefinitely, they otherwise appear normal.
The research team, led by Frederick W. Alt, a Howard Hughes Medical Institute investigator at Children's Hospital, Boston, and Harvard Medical School, published its findings in the July 2005 issue of the journal Cell Metabolism. Katrin Chua and Raul Mostoslavsky in Alt's laboratory were joint first authors of the article, which also included co-authors from the National Institutes of Health and Brigham and Women's Hospital.
The researchers began studying SIRT1 because they were intrigued by published reports from other research groups that showed that the yeast version of the gene, Sir2, extends the ability of cells to replicate. Additional studies had shown that overproducing Sir2 in the cells of worms and flies increased the organisms' life span. Such studies had led some to speculate that enhancing the activity of the mammalian version, SIRT1, might also promote longevity. For that reason, pharmaceutical companies are exploring drugs that influence SIRT1 activity, said Alt, who is also scientific director of the CBR Institute for Biomedical Research in Boston.
Alt and his colleagues have been exploring the entire seven-member family of SIRT genes because of their potential regulatory roles in the immune system, genomic stability and DNA repair. The enzymes produced by SIRT genes, called deacetylases, activate a wide range of target molecules.
In their experiments, Alt and his colleagues studied the effects of suppressing SIRT1 activity in cell cultures of mouse embryonic fibroblasts. Specifically, they explored the effect of SIRT1-deficiency on the ability of the cells to divide in culture.
"We had been studying SIRT1-knockout mice, which have a number of defects," said Alt. "And when we grew embryonic fibroblasts from those mice in culture, we observed that, unlike wild-type cells that only undergo a limited number of divisions before they reach senescence, SIRT1-deficient cells continued to grow on and on very well. That was quite surprising, because the findings in yeast and other lower organisms led those in field to speculate that if you got rid of mammalian SIRT1, the cells would senesce sooner. But in fact we got the opposite result; the cells survived and didn't undergo senescence."
To confirm that it was only suppression of SIRT1 that affected the cells, and not some secondary effect caused by knocking out the gene early in development, the researchers created mouse cells in which they could switch off the gene at will. Cells from those mice also became "immortalized." But when the researchers switched SIRT1 back on, the cells again became subject to senescence.
"So, we showed that, unlike in yeast, mouse SIRT1 can function to suppress cellular longevity rather than to promote it," said Alt. "That has been a big surprise to the field since it does not fit with preconceived notions of the role of SIRT1."