The redox-active pigments responsible for the blue-green stain of the mucus that clogs the lungs of children and adults with cystic fibrosis (CF) are primarily signaling molecules that allow large clusters of the opportunistic infection agent, Pseudomonas aeruginosa, to organize themselves into structured communities, report Massachusetts Institute of Technology geobiologists at American Society for Cell Biology (ASCB) 48th Annual Meeting, Dec. 13-17, 2008 in San Francisco.
For decades, these pigments, called phenazines, have been wrongly regarded as antibiotics, generated by P. aeruginosa , to kill off the microbe's bacterial competitors in the lungs.
This new insight about the leading cause of death of people with CF suggests that the phenazine-processing machinery could become a potential target for drugs to treat P. aeruginosa infections in CF patients, says Dianne K. Newman, who heads the MIT lab, and postdoctoral fellow Lars Dietrich.
P. aeruginosa appears as a classic opportunistic infection, easily shrugged off by healthy people but a grave threat to those with CF, which chokes the lungs of its victims with sticky mucus.
"We have a long way to go before being able to test this idea, but the hope is that if survival in the lung is influenced by phenazine -- or some other electron-shuttling molecule or molecules --tampering with phenazine trafficking might be a potential way to make antibiotics more effective," explains Newman, whose lab investigates how ancestral bacteria on the early Earth evolved the ability to metabolize minerals.
Newman and Dietrich looked at phenazines from an evolutionary perspective, and using RNA arrays to probe all of the small molecules' actions, they discovered that phenazines are not mere redox-active weapons but are molecules that activate the transcription factor SoxR.