New treatments for infertility could be closer to reality, thanks to a discovery from scientists at the Université de Montréal and Maisonneuve-Rosemont Hospital Research Centre.
According to a study published in the journal Molecular Human Reproduction, the researchers have become the first to clone, produce and purify a protein important for sperm maturation, termed Binder of Sperm (BSP), which may have implications for both fertility treatments and new methods of male contraception.
"We have previously isolated and characterized BSPs from many species, such as bulls and boars," says Dr. Puttaswamy Manjunath, senior author and a professor in the departments of medicine and of biochemistry at the Université de Montréal and a member of the Maisonneuve-Rosemont Hospital Research Centre.
"We know from these studies that if this protein is missing or defective in these species, fertility is compromised. We believe that BSP is equally important in humans."
An elusive protein
Dr. Manjunath and colleagues have tried to isolate human BSPs for more than 10 years. In most mammals, these proteins are typically produced by the seminal vesicles and added to sperm at ejaculation. Yet this is not the case for humans, primates and rodents. According to Dr.Manjunath and his team, these species produce small amounts of BSPs only in the epididymis, a duct that connects the testes to the urethra.
"For a few years, we were looking in the wrong place," says Dr. Manjunath. "In addition, the minute quantities of BSP produced in humans has made it impossible to isolate and characterize."
Cloning leads to purification