Published on January 16, 2013 at 12:36 AM
GeneTex, a leading manufacturer of high-quality antibodies, is pleased to announce that they will be launching EasyBlot, a new secondary antibody reagent that eliminates the interference that has long plagued immunoprecipitation (IP) experiments when the IP and western blot primary antibodies are from the same host. IP is a technique that involves the precipitation of a protein antigen out of a solution (e.g., a cellular lysate) through the use of an antibody that specifically binds that protein. This technique can be used to isolate and concentrate a particular protein, as well as any additional proteins to which the antigen binds (i.e., co-immunoprecipitation (co-IP)). However, the heavy and light chains of the denatured IgG used for the IP often mask the signal of the protein of interest on the subsequent western blot.
GeneTex's new EasyBlot reagent is an HRP-conjugated secondary antibody that specifically reacts with the native, non-reduced primary antibody used for the western blot while largely ignoring the reduced, denatured antibody chains of the IP antibody. Using EasyBlot as a second step reagent dramatically decreases the interference caused by the heavy (~50 kDa) and light (~25 kDa) chains of the IgG used for IP, thereby efficiently eliminating any masking of your target signal. This reagent can be utilized in IPs and co-IPs using Protein A-, Protein G-, or agarose-conjugated antibodies.
"We are very excited to offer this product to our customers." says Dr. Chun-Chiang Wen , Product Quality Manager at GeneTex, Inc. "GeneTex's EasyBlot reagent has been thoroughly optimized to ensure that there will be no interference from the heavy and light chains in IP experiments."
GeneTex offers EasyBlot reagents that detect primary antibodies from rabbit, mouse, rat, goat and sheep hosts.
SOURCE GeneTex, Inc.