LMU study shows crucial role of microRNAs in protecting precursors of neurons

Programmed cell death is an integral part of embryonic development. Exploring the regulation of the process, Ludwig-Maximilians-Universitaet (LMU) in Munich researchers have shown that so-called microRNAs protect the precursors of neurons from 'precocious' elimination.

Programmed cell death - apoptosis - is a fundamental and tightly regulated process that occurs in all higher organisms. It is, for example, essential for normal embryonic development, during which superfluous cells must be disposed of in an orderly fashion. Barbara Conradt (Professor of Cell and Developmental Biology at LMU) and her team have been studying the role of apoptosis during embryogenesis in the nematode worm Caenorhabditis elegans, a well-established experimental model. They have now shown that small fragments of RNA, so-called microRNAs, have a crucial function in the regulation of apoptosis in this system - they are responsible for the preservation of the progenitors of the nervous system. The new study appears in the journal Genes and Development.

During C. elegans embryogenesis, a stereotypic series of cell divisions give rise to the progenitor cells whose descendants go on to differentiate and form specific tissues and organs, such as the nervous system. In all, 1090 cells are produced, of which 118 are eliminated before the worm hatches from the egg. The demolition of these specific cells begins with the activation of the gene egl-1, which codes for a so-called Bcl-2-like protein and which promotes apoptosis. The coding sequence of egl-1 in the nuclear DNA is then transcribed into messenger RNA molecules. These are exported from the nucleus into the surrounding cytoplasm, and delivered to the ribosomes, which translate the information into the corresponding EGL-1 protein. "We have been able to show that certain microRNAs inhibit this process by triggering the destruction of egl-1 mRNAs," Conradt says. "This ensures that no progenitor cell is killed before it has had time to produce the daughter cells required to perpetuate its lineage."

microRNAs are short RNA fragments that have a significant role in the regulation of gene expression. They bind to specific mRNAs on the basis of their nucleotide sequence, thus preventing them from binding to ribosomes and marking them for degradation. "We were able, for the first time, to quantitatively determine - at the single-cell level - how particular microRNAs affect the levels of a target mRNA during the course of embryonic development," Conradt explains. "That is unique about our study." The progenitor cells investigated divide to give rise to daughter cells that differ in size. The smaller daughter undergoes programmed cell death, while the larger differentiates into a neuron. When Conradt's team genetically blocked the production of specific microRNAs, the level of egl-1 mRNA in progenitor cells - and thus presumably the amount of the killer protein produced - rose significantly. "Depending on the cell lineage and the nature of the mutation used, up to 80% of the precursor cells died before they could divide," says Conradt.

Under normal circumstances, when the precursor cell has divided, the functional microRNAs inherited by the smaller daughter cell are rapidly inactivated. Within minutes, the level of egl-1 mRNA rises sharply, enabling large amounts of the killer protein to be synthesized - and within 30 minutes the cell is dead. "How the inactivation of microRNAs is regulated during this rapid transition is not yet known," says Conradt. - So it's a safe bet that the spatial and temporal regulation of microRNAs will be the focus of future studies from the Conradt lab.