Discussing proliferation, outgrowth, and cell viability in picodroplets

The quick encapsulation of individual cells in miniature, picoliter-volume, water-in-oil droplets (picodroplets) is the foundation of the revolutionary single-cell analysis method known as picodroplet technology.

This strategy makes it possible to conduct research at the single-cell level much more effectively than with conventional approaches1 by enabling high-throughput screening, gentle cell processing, and high-sensitivity quantitative tests.

The capacity to screen individual cells in their natural microenvironment with better accuracy and sensitivity is made possible by the use of picodroplets, which function as small test tubes.

With the help of this format, cells can be protected from shear stress during subsequent analysis and preserved in a unique protective environment to promote their viability and integrity during incubation.

Leading pharmaceutical organizations are already utilizing this strategy to improve the workflows for antibody identification and cell line development and to hasten the discovery of new biotherapeutics after realizing the promise of picodroplet technology.

However, a broader range of other fields and applications, including cell therapy and genome editing, can benefit from picodroplet technology.

Dilution is reduced when a cell is contained within a picodroplet, and chemicals generated by the cell will accumulate and reach a measurable concentration in a comparatively short amount of time.

This often happens in applications like antibody discovery after 1–4 hours of incubation. Such downsizing might enhance the transport of reagents and aid in the identification of molecules produced by individual cells after a successful editing event in genome-editing applications.

Picodroplet compartmentalization guarantees that genetic editing occurs on a single cell as opposed to a large population of cells. Additionally, compartmentalization allows for easier detection of low-frequency events, supporting applications such as the sorting of rare genome-edited cells.

Therefore, picodroplet applications for genome editing can offer a number of benefits. For it to work, however, long-term cell incubation in picodroplets is necessary.

This article illustrates the viability and adaptability of picodroplet technology for uses requiring extended incubation durations.

Aims and objectives

The study outlined in this article demonstrates that:

  • Different cell lines can survive in picodroplets for various incubation periods
  • Suspension cells thrive in picodroplets
  • After regrowth from picodroplets, single cells can form colonies

Method

A series of experiments were carried out to evaluate if picodroplets support cell viability, proliferation, and consequential clonal outgrowth of single cells following a 24-72 hour period of encapsulation in picodroplets.

In parallel testing, seven distinct cell lines — HCT116, MCF7, A431, A549, and HEK293 for adherent lines and Raji, THP1, Jurkat, and CHO-K1 for suspension lines — were used as models for both suspension and adherent cells.

Single cell encapsulation

The input cell concentration (2e5 cell/ml) was selected to maximize the number of picodroplets that would subsequently be generated, consisting of a single cell.

Using PicoSurf 1 surfactant and a Pico-Gen 1 Biochip, cells were suspended in culture media and enclosed in 450 pL picodroplets. The emulsion was then collected in a 1.5 mL Eppendorf tube and incubated in a CO2 incubator.

Four independent time points — after cell encapsulation (time 0) and after 24, 48, and 72 hours — were used to examine the vitality and proliferation of the cells.

Cell viability

Acridine Orange and DAPI staining were used to determine the viability of the cells (Acridine Orange stains all live and dead cells, allowing for a complete cell count, whereas DAPI will only stain the dead cells).

Pico-Break, a reagent that lowers picodroplet stability, was used to break the picodroplets at each time point after a sample of the emulsion was obtained. After then, a portion of this cell suspension was dyed with Acridine Orange and DAPI and examined with a Nucleocounter NC-3000 (Figure 1).

Based on this study, a portion of the material that had not been stained was planted in 96-well plates to be examined for outgrowth.

Discussing proliferation, outgrowth, and cell viability in picodroplets

Figure 1. Cell viability in picodroplets. Image Credit: Sphere Fluidics

As shown in Figure 1, after 24 hours, all but one line (A549) still showed viability over 90%. At 48 hours, more lines’ viability began to fall, with adherent lines A549, A431, and MCF7 suffering the most.

The pattern is still present after 72 hours of picodroplet incubation, with viability from the A549, A431, and MCF7 cells dropping below 40%; Raji, HCT116, and Jurkat cells exhibit vitality around 60%; while THP1, CHO-K1, and HEK 293 cells show the viability of 80% and higher.

These studies showed that cell viability is influenced differentially depending on the cell line after picodroplet encapsulation and up to 72 hours of incubation, with suspension lines often outliving adherent ones.

However, the majority of cell lines showed a respectable capacity for survival in picodroplets for 24–48 hours, suggesting the potential viability for applications requiring extended incubation. It can be said that the lack of nutrients is mostly to blame for the observed decline in cell viability.

Cell proliferation

To establish if proliferation took place within the picodroplets, the concentration of surviving cells was compared to the initial concentration using the count from the cell viability experiment.

The outcomes showed a rise in cell concentration, notably for the suspension cells Raji, THP1, Jurkat, and CHO-K1, proving that picodroplets can promote specific cell lines’ potential for proliferation (Figure 2).

Discussing proliferation, outgrowth, and cell viability in picodroplets

Figure 2. Cell proliferation in picodroplets. Image Credit: Sphere Fluidics

Discussing proliferation, outgrowth, and cell viability in picodroplets

Figure 3. Cell outgrowth after incubation in picodroplets. Image Credit: Sphere Fluidics

This possibility could serve as a basis for further method development that will enable the generation of a “clonal population” already inside the picodroplet.

Download the full paper

About Sphere FluidicsSphere Fluidics

Our Vision

Our philosophy is simple. We combine our knowledge and resources to help you find rare and valuable biological variants, while helping you to save time, reduce costs and stay a step ahead of the competition.

Our novel single cell analysis systems offer the rapid screening and characterization of single cells. These systems are underpinned by our patented picodroplet technology, specifically designed to increase your chances of finding that rare ‘one-in-a-billion’ molecule or cell that could be an industry blockbuster.

We understand that time is of the essence. That’s why our technologies boost throughput and assay sensitivity across a range of applications. Most importantly, our flexible systems evolve alongside your changing research needs, providing an adaptable platform that helps you to meet your goals.

Our History

Founded in 2010, Sphere Fluidics is an established Life Sciences company, originally spun out from the University of Cambridge. We initially developed 25 patented products – biochips and specialist chemicals – which currently assist hundreds of customers globally with their research.

We initially focused on producing novel biochip systems and providing R&D services. We have since extended our expertise and are developing a technology platform that enables discovery in a range of growing markets through single cell analysis. Our systems make the development of new biopharmaceuticals faster and more cost-effective, improve monoclonal antibody screening, cell line development, and overall research efficiency in a number of other applications including synthetic biology, single cell diagnostics, prognostics and single cell genome editing.

The Cyto-Mine® Single Cell Analysis System is our flagship product – the first integrated, benchtop system to automatically analyse, sort and dispense millions of individual cells in just a single day.

Our Partnerships

We value and are always open to discussing new collaborative, successful and innovative academic and industry partnerships to further develop and improve our single cell technologies.

Our Technology Access Programmes and Collaborative Services exist to enable academic researchers and companies alike to tap into our application-specific expertise through direct partnerships.


Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.

Last updated: Jul 2, 2024 at 12:03 PM

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Sphere Fluidics. (2024, July 02). Discussing proliferation, outgrowth, and cell viability in picodroplets. News-Medical. Retrieved on October 31, 2024 from https://www.news-medical.net/whitepaper/20230316/Discussing-proliferation-outgrowth-and-cell-viability-in-picodroplets-1.aspx.

  • MLA

    Sphere Fluidics. "Discussing proliferation, outgrowth, and cell viability in picodroplets". News-Medical. 31 October 2024. <https://www.news-medical.net/whitepaper/20230316/Discussing-proliferation-outgrowth-and-cell-viability-in-picodroplets-1.aspx>.

  • Chicago

    Sphere Fluidics. "Discussing proliferation, outgrowth, and cell viability in picodroplets". News-Medical. https://www.news-medical.net/whitepaper/20230316/Discussing-proliferation-outgrowth-and-cell-viability-in-picodroplets-1.aspx. (accessed October 31, 2024).

  • Harvard

    Sphere Fluidics. 2024. Discussing proliferation, outgrowth, and cell viability in picodroplets. News-Medical, viewed 31 October 2024, https://www.news-medical.net/whitepaper/20230316/Discussing-proliferation-outgrowth-and-cell-viability-in-picodroplets-1.aspx.

Other White Papers by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.