Gene Expression Techniques

By Dr Ananya Mandal, MD

Gene expression is a highly regulated mechanism that controls the function and adaptability of all living cells including prokaryotes and eukaryotes. Several techniques exist for studying and quantifying gene expression and its regulation. Some of these techniques are old and well established while others are relatively new, multiplex techniques.

The field of gene expression analysis has undergone major advances in biomedical research. Traditional methods focused on measuring the expression of one gene at a time and not in any particular biological context. However, today, mRNA expression techniques have led to improvements in gene identification and disease sub-classification, for example.

Gene expression techniques

Analytical methods may be used to examine mRNA expression levels or differential mRNA expression.

Some examples of these techniques are listed below:

  • Techniques that are low- to mid-plex include:
    • Reporter gene
    • Northern blotting
    • Western blotting
    • Fluorescent in situ hybridization
    • Reverse transcription polymerase chain reaction (RT-PCR)
  • Techniques that are higher plex include
    • SAGE
    • DNA microarray
    • RNA Seq
    • Tiling array

In most cases, gene expression analysis involves both DNA and RNA quantification and a combination of these methods.

Reviewed by , BSc

Further Reading

Last Updated: Jun 24, 2014

Read in | English | Español | Français | Deutsch | Português | Italiano | 日本語 | 한국어 | 简体中文 | 繁體中文 | Nederlands | Русский | Svenska | Polski
Comments
The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News-Medical.Net.
Post a new comment
Post
You might also like... ×
Blood gene expression profile identifies patients with elevated risk of cardiovascular death