Human antibodies that thwart the SARS virus in mice can be mass-produced quickly using a new laboratory technique developed by an international research team collaborating with the National Institute of Allergy and Infectious Diseases (NIAID).
The new technique could become an important tool for developing a cocktail of SARS-specific antibodies that might help protect people recently exposed to the SARS virus or at high risk of exposure. The technique could also make possible the development of a similar approach to prevent or treat other illnesses, such as HIV/AIDS and hepatitis C.
The report describing these findings appears in the July 11, 2004, online issue of Nature Medicine.
"While much has been accomplished in our quest for a vaccine against SARS, a vaccine may provide little benefit to someone already infected," says Anthony S. Fauci, M.D., director of NIAID. "Human SARS antibodies could offer a double benefit: they could be used as a potent frontline defense for health care workers and others at high risk of exposure and as an effective treatment for those individuals newly exposed to the virus." Currently, there is no specific effective treatment for SARS.
SARS is caused by a coronavirus, a family of viruses named for their spiky, crown-like appearance. Highly contagious, SARS typically begins with flu-like symptoms, such as fever, headache and muscle aches, and generally progresses to pneumonia. In the 2003 global outbreak, more than 8,000 people were infected with SARS, 9 percent of whom died. In April 2004, a small outbreak in China is suspected to have begun as a result of negligent laboratory practices.
In the current study, Elisabetta Traggiai, Ph.D., and Antonio Lanzavecchia, M.D., from the Institute for Research in Biomedicine, Bellinzona, Switzerland, together with an international research team, generated human antibodies against SARS far more quickly and efficiently than with current methods. Moreover, collaborators Kanta Subbarao, M.D., and Brian Murphy, M.D., both in NIAID's Laboratory of Infectious Diseases, demonstrated for the first time that these human SARS antibodies, when injected into mice, effectively prevent the virus from multiplying in the respiratory system.
"The antibodies from people who have recovered from SARS may target different parts of the virus than antibodies generated by other animals, such as mice," says Dr. Subbarao. "For this reason, human antibodies--antibodies from recovered patients that may have a proven effectiveness in fighting the disease--are considered most desirable for a possible serotherapy against SARS."
Antibodies are made by special immune system cells called B cells that, to do their job, must first be switched on. In nature, this occurs when the body encounters a new or repeat foreign "invader." In the laboratory, researchers conventionally accomplish this by exposing the B cells to Epstein Barr virus (EBV), a herpes virus that infects B cells, which in turn activates them. Unfortunately, this process is very inefficient, and only one or two B cells out of one hundred are activated this way.