For many years, DNA and proteins have been viewed as the real movers and shakers in genomic studies, with RNA seen as little more than a messenger that shuttles information between the two.
But researchers from Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology have discovered that small RNA molecules called microRNAs regulate thousands of human genes--more than one third of the genome's protein-coding regions. In other words, a class of molecule once relegated to the sidelines may be one of the principal players in regulating cellular mechanisms.
"It's exciting to see how many genes are regulated by microRNAs. We now know that this type of gene control is much more widespread than previously appreciated," says Whitehead Member and MIT professor of biology David Bartel.
MicroRNAs interrupt a gene's ability to make protein. These tiny, single-stranded pieces of RNA are newcomers to biological research. It wasn't until 2000 that researchers even knew that microRNAs existed in humans. Now, in the January 14 edition of the journal Cell, Benjamin Lewis, a graduate student working jointly with Whitehead's Bartel and MIT associate professor of biology Christopher Burge, provides the first evidence that microRNAs influence a large percentage of life's functions.
The team developed a computational method to define the relationship between microRNAs and their target genes. In December 2003, the same group identified 400 genes in the human genome targeted by microRNAs. (Prior to this study, there were no known microRNA targets in any vertebrate.)
In their latest paper, taking advantage of the most recent genome-sequencing data, the team has compared human genome data with that of the dog, chicken, mouse, and rat. For each of the microRNAs and protein-coding genes that are common to these five species, the team looked for correspondence between the microRNAs and the protein-coding genes. They discovered that regulation of a third of these genes has been preserved since the last common ancestor of mammals and chicken, which lived 310 million years ago. "This study is an excellent example of the power of comparative genomics to illuminate how human genes are regulated," says Burge.