The first evidence of cardiac progenitor cells – rare, specialized stem cells located in the newborn heart of rats, mice and humans – has been shown by researchers at the University of California, San Diego (UCSD) School of Medicine. The cells are capable of differentiation into fully mature heart tissue.
Called isl1+ cells, these cardiac progenitor cells are stem cells that have been programmed to form heart muscle during fetal growth. Until this new discovery, the cells were thought to be absent after birth. However, the UCSD team discovered a small number of the specialized stem cells remained embedded in a region of the newborn heart called the atrium. They also determined that the cells could be expanded into millions of progenitor cells by growing them on a layer of neighboring heart cells called fibroblasts.
Published in the February 10, 2005 issue of the journal Nature, the research identified the isl1+ progenitor cells in the tissue of newborn rats and mice, and then in heart tissue taken from five newborn human babies undergoing surgery for congenital heart defects.
Study author Sylvia Evans, Ph.D., a member of the UCSD Institute of Molecular Medicine (IMM) and professor of pharmacology, and co-first author Alessandra Moretti, Ph.D., IMM member, explained that the cells are programmed to become spontaneously beating cardiac muscle cells simply by exposure to other neighboring heart cells.
And, since these rare cardiac progenitor cells are found in regions of the atrium that are normally discarded during routine cardiac surgery, the discovery raises the possibility that an individual could receive their own cardiac stem cells to correct a wide spectrum of pediatric cardiac diseases, according to co-first authors Moretti and Karl-Ludwig Laugwitz, M.D., a Heisenberg-Scholar of the German Research Foundation.
"Conceptually, these cells could provide a cell-therapy based approach to pediatric cardiac disease, which is new for cardiology," said the study's senior author, Kenneth Chien, M.D., Ph.D., director of the UCSD Institute of Molecular Medicine. "Traditionally, pediatric cardiologists and cardiac surgeons have relied on mechanical devices, human and synthetic tissue grafts, and artificial and animal derived valves to surgically repair heart defects. While progenitor cells won't grow a whole new heart, our research has shown that they can spontaneously become cells from specific parts of the heart by simple co-exposure to other heart cells, which could augment existing surgical procedures. If the cells maintain pacemaker function when placed in the intact heart, they might serve as biological pacemakers for infants born with heart block, which could also be valuable."
After the isl1+ cells were found in newborn rats, the UCSD team used sophisticated genetic methods to tag the progenitor cells in living embryonic tissue and in the newborn heart of mice. With these techniques, they were able to show that the isl1+ progenitor cells were spontaneously able to form cardiac muscle tissue.