Immune system cells are connected to each other by an extensive network of tiny tunnels that, like a building's hidden pneumatic tube system, are used to shoot signals to distant cells.
This surprising discovery, being reported by two University of Pittsburgh School of Medicine researchers in the September issue of the journal Immunity, may explain how an immune response can be so exquisitely swift. The research not only proves cells other than neurons are capable of long-distance communication, but it reveals a hereto-unknown mechanism cells use for exchanging information.
Blood-derived dendritic cells and macrophages, both antigen-presenting cells, make use of these so-called tunneling nanotubules to relay molecular messages, report Simon C. Watkins, Ph.D., and Russell D. Salter, Ph.D. Further research may show there are additional cell types with these microscopic tunnel connections. Thus far, their studies suggest the tunnels do not exist between commonly used fibroblast and tumor cell lines.
Interestingly, if not for a minor mishap while carrying out an experiment, the authors might not have discovered the existence of these physical structures and conducted the studies that revealed their role in intercellular communication.
Using a custom-built, multi-camera live cell microscopic imaging system, they report that, in a matter of seconds, dendritic cells and macrophages can send waves of calcium and other small molecules to cells hundreds of micrometers away. Each nanotubule measures between 35 and 200 nanometers across – 5000 times smaller than the width of a human hair – and at any given time, cells may have up to 75 of these extensions, each of varying lengths.
"Considering their scale, these nanotubules are allowing communication between fairly distant cells. If instead of a culture dish we were talking about a large metropolitan area, the distance would be about the equivalent to four or five city blocks. That's nothing short of amazing," remarked Dr. Salter, associate professor of immunology at the University of Pittsburgh School of Medicine.
The authors are the first to explain the function of tunneling nanotubules, structures that were first described in fruit flies in 1998, and subsequently, identified in a handful of different types of animal and human cells.
"It's one thing to find that this intricate physical network exists but quite astonishing to learn that immune system cells are using it to relay molecular signals to one another," said Dr. Watkins, professor and vice chair, department of cell biology and physiology, and director of the Center for Biologic Imaging, University of Pittsburgh School of Medicine.
While gap junctions – interconnecting molecular bridges that conjoin tightly packed cells – are known to generate calcium signals and transport other molecules between cells, the researchers say the tunneling nanotubules are something quite different.
"This is clearly a third form of intercellular communication, distinct from gap junctions and synapses used by nerve cells. And, it is possible that tunneling nanotubules are essential for the function of the immune system, just as gap junctions are critical for the function of cardiac muscle. Exactly how this is so, we don't know," added Dr. Watkins, who also is a professor of immunology.
"Further study may help us better understand how they're involved in the local inflammatory response of the immune system. For instance, we may find that dendritic cells use this network to distribute antigens to other cells and it may be conceivable to follow the entire pathway by tracing the network of tunneling nanotubules," said Dr. Salter.
The authors' discovery builds on their recent research showing how dendritic cells respond to stimuli, but, as they freely admit in this paper, it was due in large part to an accidental observation, that giving just the slightest poke to a single cell can set off a chain reaction whereby cell after cell discharges bursts of calcium.
In their earlier studies, they described how dendritic cells unfurl hidden veils – membranes that are so thin they can barely be imaged – and use these veils to move in on and capture their target. In the presence of E. coli, this occurs so rapidly and with such vigor that in accelerated time-lapse video, the cells appear more like a pack of wild animals feeding on a carcass.
But two things baffled the researchers. Dendritic cells extended their veils even before making physical contact with E. coli, yet macrophages, cells not normally picky about the antigens they engulf, were completely unresponsive to the bacteria. In order to understand how dendritic cells first sense the presence of an antigen and why the reaction is cell-specific, the authors decided to look at calcium flux, a well-recognized early measure of stimulation in numerous cell types. The use of a fluorescent dye, which allows direct measurement of calcium levels, would determine if calcium flux occurs before dendritic cells unfurl their veils.