X chromosome dosage compensation does occur in germ cells. A study published today in the open access journal Journal of Biology reveals that expression of the genes on the X chromosome is doubled in Drosophila germ cells to compensate for the missing second X chromosome.
The study shows that this also occurs in C. elegans and mice somatic cells. In human female somatic cells, one of the two X chromosomes is inactivated to equilibrate expression between the sexes, but this unbalances the expression of the X compared to the other chromosomes. This study is the first demonstration that the X chromosome is upregulated in germs cells, which brings the X chromosome and the other chromosomes back into balance. The study is also the first demonstration that upregulation of the X chromosome in somatic tissue is conserved across species.
Vaijayanti Gupta from the National Institute of Diabetes and Digestive and Kidney Disease at the National Institutes of Health (NIH), Bethesda, USA collaborated with colleagues from the Center for Information Technology at the NIH and colleagues from Incyte Genomics, Palo Alto, USA. Gupta et al. carried out microarray analyses of 2,245 genes present on the X chromosome of Drosophila germ cells and somatic cells. Drosophila males have one X chromosome and two autosomes (X;AA) while Drosophila females have two X chromosomes and two autosomes (XX;AA).