A University of Arkansas postdoctoral researcher has received funding from the American Heart Association to study the genetics of a fungus that has the potential to kill people when they are most vulnerable - after organ transplants.
Kristin McCue, a researcher in biological sciences in the J. William Fulbright College of Arts and Sciences, received $85,000 over two years to study the genetics of Candida albicans, a fungus commonly known for causing vaginal yeast infections in women, certain types of skin infections and diaper rash. In addition, it can also cause life-threatening infections in its systemic form, which can happen in hospitals, particularly with patients who have undergone organ transplants or other major surgery.
“This disease can travel through the blood stream and infect internal organs,” McCue said. The American Heart Association funds basic research into blood diseases, such as McCue's project.
By examining the properties of specific genes in the organism, McCue and biological sciences professor David McNabb hope to find ways to retard the growth of Candida, or render it more susceptible to antifungal medications. This could lead to better therapies for the infection.
The researchers have focused on a complex of four proteins within the organism. This complex is required for the binding of DNA – without it, the organism could not replicate or grow. One of the proteins in this complex has two different genes that code for it – an unusual trait.
“We suspect that different conditions turn on the different genes,” McCue said. “I'm trying to see which gene is needed under which conditions.”
To do this, McCue has engineered two different Candida strains, one where she deleted one of the genes that codes for this protein, and one where she deleted the other gene and kept the first one. Then she is growing the two strains under different conditions, one of which is under high and low iron conditions. The organism lives in both low iron conditions in the gut, and high iron conditions in the blood.