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Regulation of the retinoic acid gradient in zebrafish embryos

Published on November 21, 2007 at 12:39 PM · No Comments

Human embryos that get too much or too little retinoic acid, a derivative of Vitamin A, can develop into babies with birth defects.

New research published this week in the open-access journal PLoS Biology shows for the first time how embryonic cells may regulate levels of retinoic acid, providing insight into how retinoic acid signaling interacts with other key developmental signals to control development of the brain, limbs, and many other tissues in embryos.

Thomas Schilling, Richard White, Qing Nie, and Arthur Lander of University of California Irvine studied the behavior of retinoic acid in zebrafish embryos. Within a certain range, cells can regulate levels of retinoic acid. Schilling and his colleagues found that if the level becomes too high, an enzyme called Cyp26a1 degrades the excess, bringing the concentration back to normal. When levels drop too low, proteins called fibroblast growth factors (Fgfs) stop the retinoic acid from degrading as rapidly.

“Those two things work together to keep the whole system adjusted to the right level,” Schilling said. “Retinoic acid induces its own degradation, and Fgfs, also present in the embryo, have the opposite effect by inhibiting retinoic acid degradation. If you don't get enough Vitamin A in your diet—or if you get too much—your body compensates for that. Our study helps explain how this regulation occurs.”

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