Scientists have genetically engineered a mosquito to release a sea-cucumber protein into its gut which impairs the development of malaria parasites, according to research out today (21 December) in PLoS Pathogens.
Researchers say this development is a step towards developing future methods of preventing the transmission of malaria.
Malaria is caused by parasites whose lives begin in the bodies of mosquitoes. When mosquitoes feed on the blood of an infected human, the malaria parasites undergo complex development in the insect’s gut. The new study has focused on disrupting this growth and development with a lethal protein, CEL-III, found in sea cucumbers, to prevent the mosquito from passing on the parasite.
Human blood infected with malaria contains parasitic gametocytes – cells which can create parasite sperm and eggs in the gut of the insect. These then fertilise, kick-starting the parasite reproductive process and life cycle by producing invasive offspring called ookinetes.
These ookinetes then migrate through the mosquito’s stomach wall and produce thousands of ‘daughter’ cells known as sporozoites. After 10-20 days these are ready in the salivary glands to infect another human when the mosquito takes a subsequent blood meal.
The international team fused part of the sea cucumber lectin gene with part of a mosquito gene so that the mosquito would release lectin into its gut during feeding. The released lectin is toxic to the ookinete and therefore kills the parasite in the mosquito’s stomach.
In laboratory tests the research team showed that introducing lectin to the mosquito’s gut in this way significantly impaired the development of malaria parasites inside the mosquito, potentially preventing transmission to other people. Early indications suggest that this sea cucumber protein could be effective on more than one of the four different parasites that can cause malaria in humans.