A multidisciplinary research team at Case Western Reserve University led by
Gary Landreth, Ph.D., a professor in the School of Medicine's Department of
Neurosciences, has uncovered a common genetic pathway for a number of birth
defects that affect the development of the heart and head. Abnormal development
of the jaw, palate, brain and heart are relatively common congenital defects and
frequently arise due to genetic errors that affect a key developmental
pathway.
The research, titled “Mouse and human phenotypes indicate a critical
conserved role for ERK2 signaling in neural crest development” is published in
the November 10 issue of the Proceedings of the National Academy of Sciences
of the United States of America .
Landreth, also the senior author of the study, developed a mouse model of
these disorders by removing a gene central to this developmental pathway, called
ERK2. He, together with Dr. William Snider at the University of North Carolina,
discovered that the mice missing the gene for ERK2 in neural crest cells had
developmental defects resembling those of human patients with a deletion that
includes this gene. The patients have features that are similar to DiGeorge
syndrome, which is associated with cardiac and palate defects. Interestingly,
the ERK2 gene is central to a well-known pathway already associated with a
different distinct group of cardiac and craniofacial syndromes that include
Noonan, Costello, Cardiofaciocutaneous syndrome, and LEOPARD syndrome.
Landreth enlisted the help of Michiko Watanabe, Ph.D., professor of
Pediatrics at Case Western Reserve University School of Medicine to look at the
mouse hearts. She and her team found that they had characteristic heart defects
resembling those seen in the patients with ERK2 deletions.
“Given Dr. Watanabe's findings, we determined that we had in fact developed
animal models that mimicked the human deletion syndrome,” said Landreth. “This
work sheds light on how these developmental errors occur.”
Remarkably, Dr. Sulagna Saitta, a human geneticist at Children's Hospital of
Philadelphia had identified children who had comparable heart defects as well as
subtle facial differences. These children were all missing a very small region
of chromosome 22 that contained the ERK2 gene.
Saitta agreed that the similarity in the anatomic structures affected in the
mice and those in the patients who have lost one copy of this gene suggest that
ERK2 and its pathway members are essential for normal development and might lead
to these birth defects. These findings link together several distinct syndromes
that are each characterized by cardiac and craniofacial abnormalities and show
that they can result from perturbations of the ERK cascade.
Landreth and his team will take these findings back to the lab and find out
exactly why cells need ERK2 during embryogenesis.
Funding was provided by the National Institutes of Health, the National
Science Foundation, the National Heart Lung Blood Institue and a National
Research Service Award.