Cell-cycle arrest biomarkers validated for acute kidney injury

Saeed A. Jortani, Ph.D., associate clinical professor in the University of Louisville's Department of Pathology and Laboratory Medicine, headed up one of three labs in the United States involved in determining two new markers for acute kidney injury (AKI). The research group's paper, "Validation of Cell-Cycle Arrest Biomarkers for Acute Kidney Injury Using Clinical Adjudication," was posted online Feb. 25 by the American Journal of Respiratory and Critical Care Medicine.

AKI has been difficult to diagnose and treat early because current markers for it don't show up until several hours after it has begun. The research group, however, validated two new markers - tissue inhibitor of metalloproteinases (TIMP)-2 and insulin-like growth factor binding protein 7 (IGFBP7) - in urine that, when assessed together, give clinicians the ability to detect and begin treating AKI much earlier than the current standards.

The Jortani Clinical Trials Laboratories (JCTL) at UofL were among the independent labs that tested the results of the trial. The other two were at the University of California-San Diego and ARUP Laboratories, Salt Lake City, Utah.

The JCTL is certified under the Clinical Laboratories Improvement Amendments (CLIA) of the Centers for Medicaid & Medicare Services. As a CLIA-certified lab, the JCTL has undergone a rigorous review of its facilities and processes to ensure quality in the laboratory testing and analyses it provides.

AKI was formerly known as acute renal failure. It is an abrupt loss of kidney function, usually occurring within 48 hours or less. It can occur after serious infections, major surgery or taking certain medications. In its most serious form, it can cause the patient to require dialysis and can result in death. Groups at greatest risk for AKI are the elderly, men and African Americans.

Other institutions participating in the study were:

AGH Nephrology Associates
Durham VA Medical Center
Eastern Idaho Medical Consultants LLC
George Washington University
Hennepin County Medical Center
The International Heart Institute of Montana
Loma Linda University
Louisiana State University, Shreveport
The Methodist Hospital
Montefiore Medical Center
Northwestern University
Portland VA Medical Center
Rochester General Medicine
Stat'Tech Services LLC
Summa Health System Akron City Hospital
Tampa General Hospital
University of California, Los Angeles
University of California, San Diego
University of Chicago
University of Florida
University of Maryland
University of Michigan
University of Pittsburgh Medical Center
University of Utah
Vanderbilt University
Virginia Commonwealth University