Published on June 13, 2012 at 7:42 AM
The C1 System workflow is initiated by loading a sample of
cells in solution into the C1 microfluidic chip in a single
pipetting step, then directing the C1 System to rapidly and
automatically isolate up to 96 individual cells into individual chambers
for preparation. After loading, researchers can choose an "in-process"
quality control checkpoint to verify the number of captured cells and
distinguish live from dead cells to preserve data integrity. The
workflow then proceeds with a rapid "on-chip" cell lysis without RNA
purification, reverse transcription, and preamplification without
hands-on reagent mixing and sample transfer. The final preamplified cDNA
product is thereafter harvested to collection wells for transfer to the
BioMark HD System for quantitative PCR analysis.
The C1 Single Cell AutoPrep System consists of:
C1 Single-Cell AutoPrep System that provides breakthrough
bench-top automation of isolation, lysis and preamplification
performed on single cells.
C1 Single-Cell AutoPrep Array chip that facilitates capture
and highly paralleled preparation of up to 96 individual cells.
C1 Single-Cell AutoPrep Reagent Kit, a pre-formulated
"ready to use" reagent kit that supports cell suspension, lysis and
Source: Fluidigm Corporation.