Cancer cells grow fast. That's an essential characteristic of what makes them cancer cells. They've crashed through all the cell-cycle checkpoints and are continuously growing and dividing, far outstripping our normal cells. To do this they need to speed up their metabolism.
CSHL Professor Adrian Krainer and his team have found a way to target the cancer cell metabolic process and in the process specifically kill cancer cells.
Nearly 90 years ago the German chemist and Nobel laureate Otto Warburg proposed that cancer's prime cause was a change in cell metabolism - i.e., in cells' production and consumption of energy. In particular cancer cells have a stubborn propensity to eschew using glucose as a source to generate energy. This is known as the Warburg Effect.
While metabolic changes are an important feature in the transformation of normal cells into cancer cells they are not now thought to be cancer's primary cause. Despite this, metabolic changes remain an attractive target for cancer therapy, as Krainer and colleagues show in a paper published online today in Open Biology, the open-access journal of Great Britain's Royal Society.
One difference between metabolism in cancer and normal cells is the switch in cancer to the production of a different version, or isoform, of a protein produced from the pyruvate kinase-M (PK-M) gene. The protein version produced in normal cells is known as PK-M1, while the one produced by cancer cells is known as PK-M2.
PK-M2 is highly expressed in a broad range of cancer cells. It enables the cancer cell to consume far more glucose than normal, while using little of it for energy. Instead, the rest is used to make more material with which to build more cancer cells.
PK-M1 and PK-M2 are produced in a mutually exclusive manner -- one-at-a-time, from the same gene, by a mechanism known as alternative splicing. When a gene's DNA is being copied into the messenger molecule known as mRNA, the intermediate template for making proteins, a cellular machine called the spliceosome cuts and pastes different pieces out of and into that mRNA molecule.
The non-essential parts that are edited out are known as introns, while the final protein-coding mRNA consists of a string of parts pasted together known as exons. The bit that fits into the PK-M1 gene-coding sequence is known as exon 9, while it is replaced in PK-M2 by exon 10. In this way alternative splicing provides the cell with the ability to make multiple proteins from a single gene.
Krainer, an authority on alternative splicing, previously published research on the protein regulators that facilitate the splicing mechanism for PK-M. His team showed that expression of PK-M2 is favored in cancer cells by these proteins, which act to repress splicing for the PK-M1 isoform. In the study published today the team explains that it decided to target the splicing of PK-M using a technology called antisense, rather than target the proteins that regulate the splicing mechanism.
Using a panel of antisense oligonucleotides (ASOs), small bits of modified DNA designed to bind to mRNA targets, they screened for new splicing regulatory elements in the PK-M gene. The idea was that one or more ASOs would bind to a region of the RNA essential for splicing in exon 10 and reveal that site by preventing splicing of exon 10 from occurring.