From a single-cell egg to a fully functional body: as embryos develop and grow, they must form organs that are in proportion to the overall size of the embryo. The exact mechanism underlying this fundamental characteristic, called scaling, is still unclear. However, a team of researchers from EMBL Heidelberg is now one step closer to understanding it. They have discovered that scaling of the future vertebrae in a mouse embryo is controlled by how the expression of some specific genes oscillates, in a coordinated way, between neighbouring cells. Published today in Nature, their findings highlight how important this oscillatory pattern, and its regulation, is to ensure that embryos grow up to become well-proportioned animals.
Neighbouring cells in the future vertebral column of an embryo coordinate to turn specific genes on and off in turn, thus generating a wave of gene expression similar to the 'Slide to unlock' animation on your smart phone. To study this process, and determine its impact on how the relative sizes of the future vertebrae are maintained, the researchers developed a new technique.
"Using this new assay, we were able to film this wave of gene expression in real time with high precision, and to identify whether this pattern could change according to the overall size," explains Alexander Aulehla who coordinated the study at EMBL Heidelberg. "There is a clear link: when the embryo is smaller, the number of segments formed remains the same, but each segment is smaller and the expression waves are proportionally slower."