A new imaging method for the study of insulin-producing cells in diabetes among other uses is now being presented by a group of researchers at Umeå University in Sweden in the form of a video in the biomedical video journal, The Journal of Visualized Experiments.
The developed techniques have contributed to the reasons why the research team recently received a SEK 4.3 million grant from the EU in a Marie Curie program to link together leading research teams in Europe in the field of diabetes imaging.
Professor Ulf Ahlgren and his associates at the Umeå Center for Molecular Medicine (UCMM) have subsequently elaborated the technology for biomedical imaging with optical projection tomography (OPT). Initially the method could only be used on relatively small preparations, but five years ago the scientists at Umeå were able to adapt the technology to study whole organs including the pancreas from adult mice. The present findings describe a further development of the OPT technology by going from ordinary visible light to the near-infrared spectrum. Near infrared light is light with longer wavelengths that can more easily penetrate tissue. Thereby, the developed imaging platform enables studies of considerably larger samples than was previously possible. This includes the rat pancreas, which is important because rats as laboratory animals are thought to be physiologically more similar to humans.
This adaptation, to be able to also image in near-infrared light, also means that the researchers gain access to a broader range of the light spectrum, making it possible to study more and different cell types in one organ preparation. In the article the scientists exemplify the possibility of simultaneously tracking the insulin-producing islets of Langerhans as well as the autoimmune infiltrating cells and the distribution of blood vessels in a model system for type-1 diabetes.