Researchers identify new way to influence the vital serotonin signaling system

Published on February 14, 2013 at 4:58 AM · No Comments

The candidate inhibitor chosen by the researchers was a fragment of the receptor itself - specifically, the part of the receptor where PTEN attaches. Such sub-protein structures are known as "peptides"; this one was previously dubbed "3L4F." Test-tube and cell-culture experiments showed that 3L4F boosted 5-HT2C response significantly; that it acted by binding to PTEN; and that it had no effect at all on another type of serotonin receptor, designated 5-HT2A.

Behavioral studies in laboratory rats also indicated that 3L4F increased 5-HT2C responses.

"We looked at both human cells and rats because ultimately we want to translate this research into therapeutics," said UTMB postdoctoral fellow Noelle Anastasio, lead author of the paper. "The idea of targeting these interactions to produce drug and research tools is truly new and has great potential."

The team took a step toward realizing that potential by trimming 3L4F down to a peptide roughly half its size that retains similar efficacy. Using computational molecular modeling, they determined which elements of this peptide were important to bonding with PTEN - information they will use to design smaller molecules with the same or better activity.

"We've got the basics down now, so we can use the chemistry to make new molecules that we think might be potentially useful for treatment of addictions, for example," Cunningham said. "But there's also an intense interest in figuring out the biology of this interaction between 5-HT2C and PTEN, what it means in terms of disease states like the addictions, alcoholism, depression and obesity and eating disorders. I think in a broader sense this is really going to help us understand the neurobiology of these disorders."

Source: University of Texas Medical Branch at Galveston

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