Cancer cells break down sugars and produce the metabolic acid lactate at a much higher rate than normal cells. This phenomenon provides a telltale sign that cancer is present, via diagnostics such as PET scans, and possibly offers an avenue for novel cancer therapies. Now a team of Chilean researchers at The Centro de Estudios Cient-ficos (CECs), with the collaboration of Carnegie's Wolf Frommer, has devised a molecular sensor that can detect levels of lactate in individual cells in real time.
Prior to this advance, no other measurement method could non-invasively detect lactate in real time at the single-cell level. The work, published in the open access journal PLOS ONE, is a boon to understanding how different types of cells go awry when cancer hits.
"Over the last decade, the Frommer lab at Carnegie has pioneered the use of F-rster Resonance Energy Transfer, or FRET, sensors to measure the concentration and flow of sugars in individual cells with a simple fluorescent color change. This has started to revolutionize the field of cell metabolism," explained CECs researcher Alejandro San Mart-n, lead author of the article. "Using the same underlying physical principle and inspired by the sugar sensors, we have now invented a new type of sensor based on a transcriptional factor. A molecule that normally helps bacteria to adapt to its environment has now been tricked into measuring lactate for us."
Lactate shuttles between cells and inside cells as part of the normal metabolic process. But it is also involved in diseases that include inflammation, inadequate oxygen supply to cells, restricted blood supply to tissues, and neurological degradation, in addition to cancer.
"Standard methods to measure lactate are based on reactions among enzymes, which require a large number of cells in complex cell mixtures," explained Felipe Barros, leader of the project. "This makes it difficult or even impossible to see how different types of cells are acting when cancerous. Our new technique lets us measure the metabolism of individual cells, giving us a new window for understanding how different cancers operate. An important advantage of this technique is that it may be used in high-throughput format, as required for drug development."