University of South Florida study suggests high dose needed very early in pregnancy to protect developing heart
A new animal study has found that high levels of the B-vitamin folate (folic acid) prevented heart birth defects induced by alcohol exposure in early pregnancy, a condition known as fetal alcohol syndrome.
Researchers at the University of South Florida College of Medicine and All Children's Hospital report that the protection was afforded only when folate was administered very early in pregnancy and before the alcohol exposure. The dose that best protected against heart defects in mice was considerably higher than the current dietary recommendation of 400 micrograms (0.4 milligrams) daily for women of child-bearing age.
The findings were published online earlier this month in the American Journal of Obstetrics and Gynecology.
While more research is needed, the study has implications for re-evaluating folate supplementation levels during early pregnancy, said principal investigator Kersti Linask, PhD, the Mason Professor of Cardiovascular Development at USF and Children's Research Institute/All Children's Hospital.
"Congenital heart defects can occur in the developing embryo at a time when women typically do not even know they are pregnant - 16 to 18 days following conception. They may have been drinking alcohol or using prescription drugs without realizing this could be affecting embryonic development," Dr. Linask said.
"We found that we could prevent alcohol-associated defects from arising in the mice -- provided folate was given in relatively high concentrations very early in pregnancy around conception."
In the USF study, two randomly assigned groups of pregnant mice were fed diets supplemented by folate in adjusted doses known from epidemiological studies to rescue human embryos from craniofacial birth defects. From the day after conception, one group received a high dose of folate supplementation (10.5 milligrams/kilogram) and the second received a moderate dose (6.2 mg/kg). A third control group ate a normal folate-supplemented diet (3.3 mg/kg) determined to maintain the general health of the pregnant mice, but not to rescue embryos from birth defects.
During the first week of pregnancy, the mice in all three groups were then administered injections of alcohol simulating a single binge drinking event in humans.
Following this alcohol exposure, Doppler ultrasound confirmed that 87 percent of the embryos of pregnant mice in the third group - those not receiving folate supplementation beyond what was present in their normal diets - had developed heart valve defects. The affected embryos were also smaller in size and their heart muscle walls appeared thinner.