Rice University researchers model dynamic instability of microtubules

New computer models that show how microtubules age are the first to match experimental results and help explain the dynamic processes behind an essential component of every living cell, according to Rice University scientists.

The results could help scientists fine-tune medications that manipulate microtubules to treat cancer and other diseases. Rice theoretical biophysicist Anatoly Kolomeisky and postdoctoral researcher Xin Li reported their results in the Journal of Physical Chemistry B.

Microtubules are cylinders made of 13 protein strands and are one of several components of a cell's cytoskeleton. Motor proteins walk along these bundles to deliver cargoes to various parts of a cell and to discard trash. Microtubules also play a part in cell division, movement and signaling.

Cells constantly build, destroy and rebuild these cylinders and reuse the molecular blocks like Legos. "One of the most interesting phenomena associated with microtubules is this dynamic instability," Kolomeisky said. "When you look at them in cells or in vitro, they grow and grow, and suddenly start to shrink without any change in the external conditions. Then suddenly, they start to grow again."

This instability is essential to cellular processes. "If the cell stabilizes, it dies," said Kolomeisky, a professor of chemistry and of chemical and biomolecular engineering at Rice. In fact, he said, the goal of many drugs that target microtubules aim to stabilize growth so a cell stops functioning before it can reproduce. That's important in the fight against cancer, he said.

Microtubules are considered stationary when caps form to prevent them from disassembling. "The important thing we realized is that the microtubule's dynamics -- how and when it approaches a stationary state -- could depend on initial conditions," Kolomeisky said. "If you start from a very long polymer seed, it will take longer to reach a stationary state than if you start with a very short seed."

The seeds form in the cell's microtubule-organizing center, the centrosome. Microtubule strands assemble from subunits of alpha and beta tubulin proteins that form dimers. These dimers each bind two molecules of guanosine triphosphate (GTP). Beta tubulins can selectively (and reversibly) allow GTP to hydrolyze; they take on water to become guanosine diphosphate (GDP).

While reaching for their stationary state, which can take as little as 10 seconds, microtubules are in constant flux - either in a state of "catastrophe" (shrinking) or "rescue" (growing) - until they are capped.

Dimers with GDP easily fall away from the strand, but can't pop out of the middle. So when GTP molecules at the growing end fail to hydrolyze, they effectively cap the strand. GTP "islands" in the middle can also stop a catastrophe in progress and allow the strand to start growing again. The researchers' models of single microtubule filaments were able to predict when and how unhydrolyzed islands of subunits form.

The researchers looked at seeds that have either all GTP or all GDP subunits and found each strongly influences all phases of microtubule growth. They determined that where a seed contained only GTP subunits, all possible subunit configurations came into existence during the growing phase and the rate of catastrophes increased with time, as seen in experiments. The opposite was true for seeds with GDP subunits, for which the catastrophe rate started large and decreased over time.

The research is the first reported product of a program introduced by Kolomeisky and Li earlier this year to analyze the cytoskeleton, including actin and other fibers, from the molecular level up to discover the mechanisms that dictate its behavior.

Microtubules, which can be stable for minutes or even hours, were a good first target, Kolomeisky said, because many experimentalists saw their growth, stability and dissolution as a one-way process and were hard-pressed to explain signs of shrinking along the way.

He said most theoretical investigations of microtubules have only looked at their stationary states, or assumed the breakdown of a microtubule happened all at once. "A proper description of these phenomena must take time into account," he said. "You start from a small polymer seed and see how it grows. That's what we did, and it's given us the first theoretical paper to provide a fully dynamic description of how microtubules grow."