Plant and fungal cells are rich sources of RNA, including small RNAs such as microRNA (miRNA) and small interfering RNA (siRNA), which play critical roles in gene regulation, development, and defense mechanisms.
However, isolating intact total RNA from these complex samples remains a major challenge. Traditional methods involving alcohol precipitation and organic solvents often result in significant loss of small regulatory RNAs.
Advances in purification techniques now make it possible to recover high-quality RNA from diverse plant and fungal samples without the need for hazardous organic extractions.
This eBook explores optimized methods for isolating RNA across all size ranges and highlights their impact on downstream applications like real-time PCR, Northern blotting, and expression analysis.
Download the eBook
eBook highlights:
- Challenges of total and small RNA isolation from plants and fungi.
- Key considerations for RNA extraction across size ranges.
- Advantages of spin-column chromatography over traditional methods.
- Applications of purified RNA in downstream molecular biology workflows.
Download your copy of the eBook now to improve your RNA isolation workflows and drive high-quality research outcomes.
Download the eBook
About Norgen Biotek Corp.
Norgen Biotek: Advancing science with best-in-class, scientist-backed innovations
Norgen Biotek is a fully integrated biotechnology company that focuses on providing complete workflows for molecular biology sample preparation and analysis. With a diverse portfolio of over 600 products, the company delivers high-performance, user-friendly, and cost-effective solutions.
Scientifically driven, industry trusted
Norgen kits cover a broad range of applications from collection and preservation to isolation and purification. Our expert R&D team continuously develops cutting-edge technologies that set new industry standards for RNA, DNA, protein, and exosomal isolation, ensuring superior yield, purity, and integrity from even the most challenging sample types.
Unparalleled performance
At the heart of Norgen’s success is its patented Silicon Carbide (SiC) Technology. This proprietary resin exhibits uniform binding affinity for all RNA species, regardless of molecular weight or GC content. This ensures the full diversity of small and microRNA are captured while eliminating the need for phenol extraction. This innovative technology sets Norgen kits apart from others, positioning them as leaders in RNA purification.
Comprehensive solutions for any challenge
Norgen is committed to providing high-quality kits capable of processing a wide range of sample types, from ultra-low input samples such as liquid biopsies to highly impure samples like stool or soil. Our sample collection and preservation devices for stool and saliva simplify handling by rendering samples non-infectious by preventing microbial growth and inactivating viruses, while our blood and urine preservation solutions ensure the stability of highly vulnerable cell-free nucleic acids.
To meet varying research demands, we offer multiple isolation methods including, but not limited to high-throughput and automation-ready magnetic bead-based formats. Additionally, our multiple-analyte kits enable the simultaneous purification of RNA, DNA, and proteins, maximizing data extraction from a single sample.
Norgen offers an extensive variety of TaqMan qPCR kits designed for molecular diagnostic use, including lyophilized kits for easy shipping. Library preparation kits for both DNA and RNA samples are also available to support genomic applications. Norgen recently released their EXTRAClean technology, an innovative solution that minimizes background noise while providing high-purity RNA, significantly enhancing NGS performance.
Why choose Norgen?
- Scientist-Driven Innovation – Developed by leading experts in molecular biology
- Proven Quality & Reliability – ISO 9001 and ISO 13485 certified, indicating a commitment to selling high-quality products
- Global Presence – We ship to over 150 countries and have a network of 60+ distributors
- Award Winning – Norgen Biotek Corp. was honored with the 2021 Innovative Leaders Award, and recognized as the 2024 Rapid Star Award Winner in the PCR category.
- Innovative Products – Hold more than thirty issued and pending patents for products presenting solutions for all research & clinical applications
Driven by a mission to accelerate scientific discoveries, Norgen Biotek actively supports researchers by providing educational resources, technical workshops, and application notes. Their NorBlog serves as a hub for the latest scientific discoveries, protocol optimizations, and industry trends.
Explore Norgen Biotek’s innovative solutions today and take your research to the next level.
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