The Duetta™ is the latest bench-top instrument from Horiba Scientific.
The Future of Fluorescence
Two-in-One Fluorescence and Absorbance Measurements
This groundbreaking spectrometer allows the measurement of both absorbance and fluorescence, and can also be used either merely as a fluorometer, or as a UV-Vis-NIR spectrometer for absorbance measurement.
The Duetta is designed with an automatic real-time Inner Filter Effect (IFE) correction for both Primary and Secondary IFE, which is particularly important for getting a true molecular fingerprint with simultaneous measurement.
A new fingerprinting method, called A-TEEM, integrates Absorbance-Transmission and EEM (excitation-emission matrix).
Fast and Accurate
The Duetta is fitted with a high-tech Charge Coupled Device (CCD Detector) rather than traditionally used photomultiplier tubes (PMT) for fluorescence detection.
This technology allows detection of a full fluorescence spectrum in just a millisecond. A complete 3D fluorescence EEM can be attained within a second.
The CCD detector also covers NIR wavelength detection to 1100 nm.
The complete wavelength range of the Duetta is from 250 nm to 1100 nm for UV-Vis-NIR Fluorescence detection.
The maximum level of fluorescence sensitivity allows correct measurement even for low concentration samples.
Touch-Screen Software EzSpecTM
Another Horiba Scientific innovation has been embedded in the Duetta – The touch-screen software interface having a selection of Apps for routine analysis.
- Detection range from 250 nm to 1100 nm
- Simultaneous measurement of fluorescence and absorbance
- Rapid detection with Charge Coupled Device: whole fluorescence spectrum in just a millisecond
- Real-time Inner Filter Effect correction for both Primary and Secondary IFE
- A-TEEM fingerprinting integrates Absorbance-Transmission and EEM
- EzSpecTM touch-screen software interface
||Simultaneous Absorbance and Fluorescence Spectrometer
||SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits
|Spectral Acquisition Rate
|EEM Acquisition Rate
||As fast as 1 second (sample and wavelength dependent)
|A-TEEM Acquisition Rate
||As fast as 30 seconds (sample and wavelength dependent)
||CCD / Spectrograph
|Fluorescence Detector Range
||1, 2, 3, 5,10, 20 nm (excitation and emission)
|Absorbance Detector Range
||2, 3, 5, 10 nm
||75 W Xenon arc lamp. Dedicated cartridge for snap-in replacement
|Excitation/Absorbance Wavelength Range
||+/- 0.02 A
||+/- 1 nm
||Windows® 10/8/7 Laptop or PC with 1 USB
|Dimensions (W x D x H)
||17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm
||45 pounds; 20.4 kg
|Polarizers (Time of Purchase)
||Yes (280 to 750 nm)