Duetta™ - Fluorescence and Absorbance Spectrometer

The Duetta™ is the latest bench-top instrument from Horiba Scientific.

Two-in-One Fluorescence and Absorbance Measurements

This groundbreaking spectrometer allows the measurement of both absorbance and fluorescence, and can also be used either merely as a fluorometer, or as a UV-Vis-NIR spectrometer for absorbance measurement.

The Duetta is designed with an automatic real-time Inner Filter Effect (IFE) correction for both Primary and Secondary IFE, which is particularly important for getting a true molecular fingerprint with simultaneous measurement.

A new fingerprinting method, called A-TEEM, integrates Absorbance-Transmission and EEM (excitation-emission matrix).

Fast and Accurate

The Duetta is fitted with a high-tech Charge Coupled Device (CCD Detector) rather than traditionally used photomultiplier tubes (PMT) for fluorescence detection.

This technology allows detection of a full fluorescence spectrum in just a millisecond. A complete 3D fluorescence EEM can be attained within a second.

The CCD detector also covers NIR wavelength detection to 1100 nm.

The complete wavelength range of the Duetta is from 250 nm to 1100 nm for UV-Vis-NIR Fluorescence detection.

The maximum level of fluorescence sensitivity allows correct measurement even for low concentration samples.

Touch-Screen Software EzSpecTM

Another Horiba Scientific innovation has been embedded in the Duetta – The touch-screen software interface having a selection of Apps for routine analysis.

Duetta Highlights

  • Detection range from 250 nm to 1100 nm
  • Simultaneous measurement of fluorescence and absorbance
  • Rapid detection with Charge Coupled Device: whole fluorescence spectrum in just a millisecond
  • Real-time Inner Filter Effect correction for both Primary and Secondary IFE
  • A-TEEM fingerprinting integrates Absorbance-Transmission and EEM
  • EzSpecTM touch-screen software interface

Specifications

Duetta Duetta-Bio
Acquisition Modes Simultaneous Absorbance and Fluorescence Spectrometer
Fluorescence Sensitivity SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits
Spectral Acquisition Rate 510,000 nm/min
EEM Acquisition Rate As fast as 1 second (sample and wavelength dependent)
A-TEEM Acquisition Rate As fast as 30 seconds (sample and wavelength dependent)
Fluorescence Detector CCD / Spectrograph
Fluorescence Detector Range 250-1,100 nm
Fluorescence Bandwidth 1, 2, 3, 5,10, 20 nm (excitation and emission)
Absorbance Detector Silicon Photodiode
Absorbance Detector Range 250-1,000 nm
Absorbance Bandwidth 2, 3, 5, 10 nm
Light Source 75 W Xenon arc lamp. Dedicated cartridge for snap-in replacement
Excitation/Absorbance Wavelength Range 250-1,000 nm
Absorbance Accuracy +/- 0.02 A
Wavelength Accuracy +/- 1 nm
Absorbance Range 0-2 A
Computer Requirements Windows® 10/8/7 Laptop or PC with 1 USB
Software EzSpec
Dimensions (W x D x H) 17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm
Weight 45 pounds; 20.4 kg
Polarizers (Time of Purchase) Not Available Yes (280 to 750 nm)