Enzo Life Sciences’ GFP-Certified® FluoForte® Calcium Assay Kit (high-throughput) provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, the GFP-Certified® FluoForte® dye is the brightest and most sensitive fluorescent calcium indicator. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines. In addition, it requires neither a washing step, nor exogenous addition of a quencher dye, which could adversely effect receptor-ligand interaction kinetics.
- Monitor calcium mobilization in GFP-expressing cell lines
- The brightest and most sensitive red-shifted fluorescent Ca2+ indicator
- Provides comparable EC50 and IC50 values as obtained using Rhod-4 AM and Fluo-4 AM
- Larger assay window and higher Z' factor values, allowing measurements of challenging cell lines and receptors