The MicroTime 200 from PicoQuant is the only commercial instrument that provides single molecule sensitivity and extremely high temporal resolution combined with outstanding capabilities and ease-of-use. It allows numerous measurement and analysis modes and is established as a versatile tool for current research and analysis topics.

The advances of cutting edge science in many fields depend on single molecule studies. This includes, for example, the quantification of molecular dynamics or molecular properties as well as interaction studies in material and life sciences. Such a wide field of research requires a flexible instrument, which can be adapted to the individual needs. This versatility is given in the MicroTime 200, a time-resolved confocal fluorescence microscope system. This powerful instrument is ready to analyze a multitude of parameters down to the single molecule level using methods such as Fluorescence Lifetime Imaging (FLIM), FLIM/FRET, deep tissue FLIM, PIE, FCS/FCCS, FLCS/FLCCS, dual-focus FCS, anisotropy, burst analysis, simultaneous AFM/FLIM or deep UV detection, to name only the most common. Even high resolution imaging with spatial resolutions below 50 nm is possible with the new MicroTime 200 STED add-on.


  • Complete confocal system with laser combining unit, inverted microscope body and multichannel detection unit
  • Turn-key diode lasers for multicolor excitation from 375 nm to 900 nm
  • Up to 4 truly parallel detection channels using application-optimized detection with SPADs, PMTs or Hybrid-PMTs
  • Time-Correlated-Single Photon Counting (TCSPC) and TTTR mode for investigation of fast dynamics in FCS and FLIM
  • Piezo scanning for 2D- and 3D-lifetime imaging and accurate point positioning
  • Two optional exit ports for additional hardware, e.g. spectrographs
  • Advanced easy-to-use data acquisition, analysis and visualisation software SymPhoTime 64
  • Unique upgrades to 2focus FCS, simultaneous AFM/FLIM, UV measurements
  • NEW STED add-on for high-resolution imaging


  • Single Molecule Spectroscopy
  • Time-Resolved Fluorescence
  • Fluorescence Lifetime Imaging (FLIM)
  • Phosphorescence Lifetime Imaging (PLIM)
  • Fluorescence Correlation Spectroscopy (FCS)
  • Fluorescence Lifetime Correlation Spectroscopy (FLCS)
  • Foerster Resonance Energy Transfer (FRET)
  • Dual-focus Fluorescence Correlation Spectroscopy (2fFCS)
  • Pulsed Interleaved Excitation (PIE)
  • Fluorescence Anisotropy (Polarization)
  • Pattern Matching Analysis
  • Time-Resolved Photoluminescence (TRPL)
  • TRPL Imaging
  • Antibunching