The NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer from Thermo Fisher Scientific helps to avoid costly delays and improve understanding of sample quality. The NanoDrop One/OneC Spectrophotometer comes with innovative Thermo Scientific™ Acclaro™ Sample Intelligence technology to deliver improved measurement precision and contaminant identification.

The NanoDrop One/OneC system can quantify and qualify RNA, DNA, and protein samples in seconds with only 1–2 μL. It provides complete spectral information so users can make informed decisions before using samples in downstream applications. It also comes with additional cuvette positions.

Product overview

The system supports users to advance closer to the results with an ergonomic, standalone spectrophotometer with a high-resolution, touch-screen interface. The robust auto-range pathlength technology enables precise measurements for concentrated samples with no requirement for dilutions. 

Acclaro sample intelligence technology

The Acclaro technology improves better learning of sample quality while delivering precise quantitative measurements. Early identification of contaminants limits failure of downstream applications and saves time spent in troubleshooting.

  • Enables information access through alerts to receive real-time feedback regarding sample quality with on-demand technical support and guided troubleshooting
  • Dependable result accuracy to maintain measurement integrity with embedded sensor and digital image analysis
  • Support to verify sample quality in seconds, facilitating identification of sample contaminants and achieve corrected concentration results
  • To identify nucleic acid contaminants such as RNA contamination in dsDNA samples and chemical contaminants like phenol in RNA samples

NanoDrop One/Nanodrop OneC PC software with 21 CFR Part 11 compliance

  • New PC control software enables users to control the instruments from an Ethernet-connected PC
  • Optional Thermo Scientific Security Suite for NanoDrop One/OneC facilitates the usage of an instrument in 21 CFR Part 11 compliant laboratories, and the features include:
    • Audit trail
    • User account access
    • Electronic signatures
    • Automated data back-up and more

NanoDrop One/NanoDrop OneC enhancements

  • Ergonomic and standalone design with a combined Android™ tablet prevents the need for a standalone computer and offers seamless information transfer through wireless, Ethernet, or USB connections
  • Wider dynamic range avoids the need for dilution for highly concentrated samples (dsDNA at 27,500 ng/µL) with auto-range pathlength technology
  • Auto-Measure and Auto-Blank functions enable multi-sample processing to be streamlined with real-time measurements that happen when the pedestal arm is down. It is possible to turn these functions on and off with a single tap on the screen
  • Integrated Learning Center acts as a register of technical support documents and educational animations for the user

Advanced connectivity for data storage, printing, and sharing

  • Users can view, organize, and share data on a PC: After data export, users can view the complete data formats, for example, contaminant analysis spectral graphs, and execute experiments using the NanoDrop One/OneC PC Control software
  • Multiple choices for data export: The feature enables the transfer of data with a USB, Wi-Fi, or Ethernet connection to the PC or facilitates data storage to the Thermo Fisher Cloud Connect Platform via Wi-Fi or Ethernet network
  • 24/7 access to data: View data anytime, anywhere from any device (Android-based or iOS-based devices, PC or Mac desktop computers) during the collection of data, analysis, and storage and also while sharing NanoDrop One data on the NanoDrop App found in the Cloud Connect Platform (requires registration)
  • Easy data-to-printer options: Facilitates printing of data directly from the NanoDrop One instrument through Wi-Fi, Ethernet, or USB printer connection; the Nanodrop One/OneC PC Control Software also enables printing of exported data from the PC
  • Online collaboration helps to share data between colleagues who have no access to an instrument and view NanoDrop One data across several Thermo Fisher Cloud-connected accounts

NanoDrop OneC Spectrophotometer highlights

  • Capable of measuring dilute samples, performing kinetics experiments, and carrying out optical density measurements of bacterial cultures
  • Comprises both pedestal and cuvette positions to increase experimental flexibilities and increase the dynamic range
  • Cuvette positions include temperature control and stirring

NanoDrop One/NanoDrop OneC instrument capabilities

  • Has a broad spectral range of 190–580 mm for the measurement of a variety of sample types:
    • Gold nanoparticles (520 nm)
    • Toxicology assays and industrial dyes (490 nm)
    • Peptides (205 nm)
    • Purified protein (280 nm)
    • DNA and RNA (260 nm)
    • Optical density measurements (600 nm)
    • Colorimetric protein assays (BCA 562 nm, Bradford 595 nm, Modified Lowry 650 nm, Pierce 660 nm)
  • Pedestal measurements demand only 1–2 μL of the sample and with no sample dilution, even in the case of highly concentrated samples
  • Combining the patented sample-retention system with cuvette capabilities with low and high concentrations (2.0–27,500 ng/µL dsDNA and 0.06–820 mg/mL BSA)
  • The user-friendly software has custom techniques and data exports potential
  • Ability to calculate sample purity ratios (A260/A280 nm and A260/A230 nm)
  • Pre-configured approaches for DNA, Microarray, Protein and Labels, Protein A280, Bradford, Pierce 660, BCA, and Lowry

Video Credit: Thermo Fisher Scientific – Materials & Structural Analysis

Video Credit: Thermo Fisher Scientific – Materials & Structural Analysis

Video Credit: Thermo Fisher Scientific – Materials & Structural Analysis

Video Credit: Thermo Fisher Scientific – Materials & Structural Analysis

Video Credit: Thermo Fisher Scientific – Materials & Structural Analysis

NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer

Image Credit: Thermo Fisher Scientific – Materials & Structural Analysis

NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer

Image Credit: Thermo Fisher Scientific – Materials & Structural Analysis

NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer

Image Credit: Thermo Fisher Scientific – Materials & Structural Analysis

NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer

Image Credit: Thermo Fisher Scientific – Materials & Structural Analysis

NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer

Image Credit: Thermo Fisher Scientific – Materials & Structural Analysis

NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer

Image Credit: Thermo Fisher Scientific – Materials & Structural Analysis