The IMA™ from Photon etc. is a hyperspectral microscope that offers spectral and spatial data in the VIS, NIR and SWIR ranges (from 400 to 1620 nm). This technology maps fluorescence, reflectance and transmittance very fast. IMA is quicker and more effective than typical point-by-point or line-scan-based systems thanks to its high-throughput global-imaging filters. It is useful for biological analysis and research on organic and inorganic substances.
Key features
Quick global mapping (non-scanning)
Absolute photometric calibration
Excellent spatial and spectral resolution
Non-destructive testing under real-world conditions
Extremely adaptable
Complete system (camera, illumination module, filters, microscope and software)
Photon etc - IMA Hyperspectral Microscope Play
IMA Global Hyperspectral Microscope: Video Credit: Photon etc.
Applications overview
IR markers in complex environments such as live cells and tissue can be studied, for instance, the spectral heterogeneity of IR fluorophores produced in the second biological window
Obtaining darkfield images and contrast of transparent and unstained samples like living cells, polymers or crystals
Multicolor markers tracking (deep multiplexing in vivo)
Recognizing disease-affected tissues
Cellular environment monitoring
Specific applications
Optical biosensor for detecting Escherichia coli concentrations
Using single-walled carbon nanotubes (SWCNT) as fluorescent probes for multiplexed imaging
Tracking of various labelled receptor subtypes in live neurons
Specifying isolated carbon nanotube species' molar concentrations
Mapping of endolysosomal lipid flux
Reporting of an enzymatic suicide inactivation pathway
Assessment of nitric oxide concentration
Identification and localization of gold nanoparticles (AUNP) using hyperspectral darkfield characterization
Reporting of microRNA hybridization events in vivo
Nuclear entry reporting via a noncanonical pathway
Identification of colon cancer metastasis in the liver whilst operating
Biomolecular functionalization to control live-cell stability and retention
Drug screening for CNS diseases by screening multiple protein subtypes simultaneously and live tracking of their spatial dynamics
Investigating the permeation of multicellular tumor spheroids
Ascertaining the molar concentrations of isolated carbon nanotube species
Monitoring of different receptor subtypes labelled in live neurons
Accelerated detection of Escherichia coli in phosphate-buffered saline solution
Product specifications
Source: Photon etc.
VIS SWIR
Spectral range
Spectral resolution (FWHM)
Spectral channels
Spatial resolution
Sub-micron - limited by the microscope objective NA
Sub-micron - limited by the microscope objective NA
Camera
Photon etc. InGaAs camera (ZephIR™ 1.7 or Alizé™ 1.7)
Excitation wavelengths (up to 3 lasers)
405, 447, 532, 561, 660, 730, 785, 808 nm (other wavelengths available upon request)
405, 447, 532, 561, 660, 730, 785, 808 nm (other wavelengths available upon request)
Microscope
Upright or inverted, scientific grade
Upright or inverted, scientific grade
White light illumination
Diascopic, episcopic, Hg, halogen
Diascopic, episcopic, Hg, halogen
Illumination options
Epifluorescence module, darkfield module (oil or dry)
Epifluorescence module, darkfield module (oil or dry)
Image Credit: Photon etc.