The MiSeq FGx® Forensic Genomics Solution available from Verogen is the first-ever fully validated sequencing workflow developed exclusively for human identification applications (Figure 1).

Illumina MiSeq FGx® Instrument—The Illumina MiSeq FGx® instrument is a compact, fully validated next-generation sequencing platform for forensic genomics applications.

Figure 1. Verogen MiSeq FGx® Instrument—The MiSeq FGx® instrument is a compact, fully validated next-generation sequencing platform for human identification applications. Image Credit: Verogen.

Thanks to the high resolution and superior accuracy of next- generation, or massively parallel sequencing technology, the MiSeq FGx® Solution can ensure powerful results even for degraded, mixed, or most delicate samples.

A Complete Sample-to-Answer Solution

For forensic DNA sample analysis, the MiSeq FGx® Solution offers a comprehensive DNA-to-data workflow. The solution starts with the ForenSeq™ DNA Signature Prep Kit, containing reagents needed to make DNA libraries for sequencing (Figure 2).

ForenSeq DNA Signature Prep Kit—The ForenSeq DNA Signature Prep Kit includes all reagents required to prepare DNA libraries for sequencing, including PCR reagents, index adapters, and purification and normalization beads.

Figure 2. ForenSeq DNA Signature Prep Kit—The ForenSeq DNA Signature Prep Kit includes all reagents required to prepare DNA libraries for sequencing, including PCR reagents, index adapters, and purification and normalization beads. Image Credit: Verogen.

An RFID labeled reagent cartridge, wash solution, and sequencing reagents provided in the MiSeq FGx® Reagent Kit are loaded onto the Verogen MiSeq FGx® instrument, together with the sequencing-ready DNA libraries.

The intuitive touch screen interface offers easy, stepwise guidance for every stage of the sequencing run, such as loading reagents and library, monitoring of the run, and run configuration. The hands-on time required is minimized by automating the generation of the onboard clusters and analysis initialization.

The Verogen ForenSeq™ Universal Analysis Software offers a robust set of forensic analysis capabilities, such as population statistics generation, CODIS-compatible report generation, automatic detection of mixed DNA samples, and generation of an optional offline report on  flanking region variation.

Furthermore, the software allows biogeographical ancestry and visible traits estimation, offering vital investigative leads in “no suspect” cases.

Highlights

Sample-to-answer solution that is fully validated—Comprehensive system combines the preparation of library, sequencing, data analysis, and reporting into a single integrated workflow.

Simple, streamlined workflow—200 genetic markers can be examined in a single amplification, which avoids the need for multiple STR kits.

A wider range of information can be accessed—Phenotypic-informative SNPs, sequence variants, and biogeographical ancestry offer valuable data that cannot be obtained extensively using existing technology.

Superior analysis of difficult samples—Optimized capacity to handle difficult samples such as degraded DNA or complex mixtures.