Malvern Panalytical has introduced the NanoSight Pro, which offers the most straightforward, quick, and easy solution available for the characterization of nano- and biomaterials using Nanoparticle Tracking Analysis (NTA).
Advanced engineering and a slew of clever features ensure that NTA measurements are effective, rapid, and available to all users. The NS Xplorer program, driven by machine learning, eliminates subjectivity, automates measurements, and delivers the best size and concentration data possible for fluorescence and light scatter analyses.
Thanks to interchangeable lasers, applications can be more flexible, and a Smart Manager connection ensures robustness without causing quality problems or downtime.
Overview
The software interface is logical and user friendly, even for a first time user.”
Rebecca Dragovic, Teaching Fellow & Research Scientist, Nuffield Department of Women’s & Reproductive Health, University of Oxford
NanoSight Pro: Expert Nanoparticle Tracking Analysis
Video Credit: Malvern Panalytical Ltd
Features and benefits
Quick install and analysis
Get up and running quickly with Smart Install! With minimum setup, easy operation and intuitive NS Xplorer software you can start generating actionable information and delivering value for your business in minutes.
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Intelligent and automated
Powered by machine learning and intelligent features, NanoSight Pro automates processing and eliminates human error, delivering superior particle identification and precise particle tracking for the highest quality and reproducible data.
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Highest resolution
NanoSight Pro measures nanoscale particles in the range of 10 nm - 1000 nm including low scattering and biological particles. Particle–by–particle analysis assures high resolution size and concentration data with visual confirmation.
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Power of fluorescence
In addition to light scatter, fluorescence signals can be detected from fluorescently tagged biologics or synthetic particles providing even greater insight into your sample. Interchangeable laser modules with the choice of four excitation wavelengths can be used along with the matching long pass filters for the optimum setup supported by analysis in flow for better sampling and tracking of even photobleaching samples.
The greater fluorescence sensitivity enables subpopulation identification including surface markers, internal cargo detection or depreciation of contaminants sensitivity.
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It is the easiest bit of equipment I have ever installed in the lab.”
Oliver Huseyin, Research scientist, MIP Diagnostics Limited
Connected world
Smart Manager
Unleash the potential of the data
When you connect your NanoSight Pro to our Smart Manager, you can unleash its full potential. Smart Manager continuously evaluates instrument data on the cloud, monitoring the health and utilization of your instrument. Simply connect. This is just one of our digital solutions that are part of Malvern Panalytical's Smart Manager.
Unleash the potential of your data; meet Smart Manager
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Key applications
NanoSight Pro can be used in many key applications, including:
Extracellular vesicles (EV)
Even though knowledge about the production and purposes of extracellular vesicles is still developing, it is critical to manage the purification and separation processes. While fluorescence can help clarify the origin subpopulations of EVs, NanoSight Pro offers rapid and straightforward assessment of the size and concentration of vesicles in aqueous buffers, so you can have confidence in the sample quality used in your research.
Virus and vaccines
Manufacture of vaccines requires controlled production processes to ensure that the materials can be appropriately dosed and recognized by the immune system. NTA assists in the optimization of manufacturing processes by ensuring the size distribution of the vaccine is maintained. As NTA is also able to count and size viruses, it can be used as a faster alternative to titer assays.
Drug delivery and gene therapy
With drug delivery and gene therapy products, 70-150 nm is the typical size of particles required to ensure effective disease targeting in the clinical. NTA is ideally suited to study the size of these particles from early stage research through to candidate screening, formulation development and clinical batch monitoring whilst the concentration measurement allows for dose determination of the final product as well as for use in in vitro and in vivo assays.
Biotherapeutics
Temperature, pH change, agitation, shear and time all impact the stability of biotherapeutic proteins, causing denaturation and aggregation which can cause loss of efficacy and the potential for an unwanted immune response. NTA provides high resolution size distributions of sub-visible aggregates in a formulation for safety and quality assurance.
Nanomaterials/colloids/toxicology
As nanomaterials are incorporated in to everyday items, they are coming to the attention of regulatory bodies. There is a requirement to fully characterize these particles, their properties and contribution in the final product. NanoSight can provide a number-based concentration for these nanomaterials and high-resolution size distributions for those working at the nanoscale in industrial, environmental and toxicological fields.
Ultrafine bubbles
In recent years work on ultrafine bubbles has been gathering momentum for use in industrial cleaning and water treatment, agriculture and food, and medical applications amongst others. Their relatively low concentration and small size are a challenge for many traditional nanoparticle characterization techniques whereas NTA is particularly adept at the detection and analysis of these bubbles.
Specifications
Source: Malvern Panalytical Ltd
| General |
| Technology |
Nanoparticle Tracking Analysis |
| Size range (diameter)1 |
10 nm - 1000 nm |
| Particle concentration2 |
106 to 109 particles / mL |
| Minimum sample volume |
250 μL |
| Advanced Concentration Algorithm |
Concentration Upgrade |
| System |
| Product Compliance |
Product Laser Class 1 (BS EN 60825-1:2014)
EMC Directive (EN IEC61326-1:2021)
Low Voltage Directive (IEC 61010-1:2010
IEC 61010-1:2010/AMD 1:2016) |
| Camera |
High sensitivity sCMOS (USB-3) |
| Laser information |
Beam wavelength (maximum power output):
405 nm, max power <70 mW
488 nm, max power <55 mW (blue)
532 nm, max power <60 mW (green)
642 nm, max power <50 mW (red) |
| Temperature control range |
5 °C below ambient up to 70 °C |
| Temperature readout |
Automatic |
| Syringe pump |
Continuous sample flow with 1 mL syringes |
| Dimensions (W, D, H) |
250 x 350 x 340 mm |
| Weight of instrument |
11 kg |
| Weight of laser module |
1.6 kg |
| Power requirements |
AC 110 – 240 V, 50-60 Hz, 4.0A |
| Ambient operating conditions |
Up to 80% rH at 31 °C then decreasing linearly to 50% at 40 °C |
| Additional options |
| Fluorescence – automatic selection3 |
For up to 5 filters |
1 Dependent on sample and instrument configuration
2 Sample dependent
3 Optional functionality. Long-pass filters available for each laser wavelength