AP 22 IF BLOT ELITE is a full automated system for ELISA and IFA and BLOT methods. The instrument is able to run 2 ELISA micro-plates with 8 tests on line, 16 IFA slides or 24 BLOT strips; besides of ELISA and IFA processing (AP22, AP22 IF), it is able to carry out the full automation for the Dot/Western Blot, thanks to the blot module and a camera, starting from sample dilution and dispense, reagent dispense, incubation, strip washing to the dot imaging processing and assessment for each strip; the software also provides the data record and patient report.
Technical Features
| ELISA technical features |
| ELISA dilution cups |
110 positions |
| Removable trays |
Modular rack to hold calibrators, controls and reagents for 8 tests on line |
| Microtiter plates |
2 standard plates (12 x 8 well format) incubated at room temperature or from 36° to 45 °C |
| Shaker |
Microtiter plate shaking capability (optional) |
| Liquid handling |
1 probe for dilution and dispense |
| Sample dispense time |
11 s with 2 ml of wash buffer |
| Plate washing |
Two 8 nozzles manifold, one to dispense and the second to aspirate; residual volume <3 µl |
| Wash buffers |
2 Buffers for Elisa microplates in external tanks with level sensors |
| Plate washing inaccuracy |
CV <5% with 300 µl |
| Reading system |
8 independent photo-metric channels with mono and bichromatic reading capability |
| Interferential filters |
2 precision filters (450 e 630 nm); 2 more on request (4 filters max; 400-700 nm) |
| Reading capability |
Reading from 0.000 a 3.000 OD; inaccuracy ± 1% from 0.000 to 1.500 OD ± 2% from 1.500 to 3.000 OD |
| IFA technical features |
| Liquid handling |
2 probes: one to dilute and dispense, the second for slide washing |
| Sample dispense time |
11 s with 2 ml of wash buffer |
| Slide processing time |
30 minutes for dispense or wash 16 slides with 12 wells |
| Supplied trays |
10 tests on line capability: tray with 220 positions for dilution cups; tray with 16 positions for IFA slides; reagent tray for 18 controls, 9 conjugates, 1 cleaning solution for the probe and 2 diluents for samples |
| Liquid sensor probe |
Able to detect 150 µl |
| Wash buffers |
2 IFA slides wash buffers in external tanks with level sensors |
| Slide washing modes |
each well is washed independently, continuous flow or multiwell mode for specific washing effectiveness |
| Caratteristiche tecniche BLOT |
| Liquid handling |
2 probes: one to dilute and dispense, the second for strip washing |
| Dispensation |
up to 3000 µl with 1 µl steps resolution |
| Reagent tray |
10 tests on line capability: 10 x 110 ml reagent vials and 10 control positions |
| Strip holders |
3 trays (8 strip per tray) to run up to 24 strips on the shaking plane |
| Wash buffers |
2 strips wash buffers in external tanks with level sensors |
| Reading capability |
High resolution color camera for imaging acquisition |
| COMMON technical features |
| Sample barcode reader |
built-in sliding sample barcode reader |
| Sample tray |
91 sample tubes Ø13 mm or 70 sample tubes Ø16 mm; both kinds can be used at the same time |
| Dilution cuvettes |
22 cup blocks for easy loading |
| Syringe |
1000 µl volume; 1 µl step resolution |
| Dispensation |
from 8 µl to 1000 µl by steps of 1 µl; inaccuracy ±1% at 100 µl |
| Liquid tanks |
WASH and WASTE tanks equipped with level sensors |
| Connection |
LIS interface capability, work list import from Host Computer, a single PC can run more instruments in parallel |
| Operative systems |
Windows 7 or Windows 10 |
| PC |
Minimum requirements: 250 MB free space Hard Disk; CD-Rom reader ; 2 GB RAM; Intel Pentium Dual Core or equivalent, VGA 1024 x 768 16 million colors; 2 free USB 2.0 port; printer.
Suggested requirements: 4 GB RAM; Intel Core 2 Duo E6500 series processor or equivalent |
| Power requirement |
230 / 115 VAC, 50-60 Hz, 350 W |
| Measurements |
cm 62 x 83 x 72 h |
| Weight |
85 Kg |