DeNovix's dsDNA Fluorescence Quantification Assays

The DeNovix dsDNA Fluorescence Quantification Assays provide the greatest sensitivity and widest dynamic range available today. DeNovix offer three highly specific assay kits for a wide range of sample concentrations.

DeNovix kits selectively and accurately measure dsDNA even in the presence of common contaminants such as RNA, ssDNA or protein. Combine the specificity of fluorescence with the purity measurements of absorbance on our DS-11 FX range for the optimum sample QC procedure.

Features

  • Widest Dynamic Range: 0.5 pg/µL – 4000 ng/µL
  • 20X Greater Sensitivity than Qubit®
  • Integrated into EasyApps® Software
  • Simple Mix-and-Measure Assay

Assay Choices for Your Application

DeNovix dsDNA assays cover three overlapping ranges of sensitivity. Each kit includes standards for easy set up of a two-point standard curve. DeNovix assays have a wider dynamic range than Qubit® dsDNA assays – and are more affordable.

Our breakthrough dsDNA Ultra High Sensitivity assay allows measurement of samples as low as 0.5 pg/µL (original sample concentration), 20X lower than the core range of the Qubit® dsDNA HS assay.

DeNovix dsDNA assays may be used on instruments other than the DeNovix DS-11 FX and QFX series of fluorometers. Use the Detailed Protocol links below for additional information. Click here for downloadable Qubit fluorometer .qbt files for use on Qubit 2.0 and 3.0 fluorometers.

Dynamic Range

Microplates

For those labs requiring higher throughput, all DeNovix assays are also compatible with microplate formats.

Assays

DeNovix dsDNA Broad Range Assay

Range: 0.1 ng/µL to 2000 ng/µL (extended to 4000 ng/µL) dsDNA
Kit options: 1000 assays, 250 assays or 50 assay evaluation size
Excitation: 375 nm
Emission: 435-485 nm

Dynamic Range vs. Qubit®

Dynamic Range vs. Qubit®

DeNovix dsDNA High Sensitivity Assay

Range: 5 pg/µL to 250 ng/µL dsDNA
Kit options: 1000 assays, 250 assays or 50 assay evaluation size
Excitation: 470 nm
Emission: 514-567 nm

Dynamic Range vs. Qubit®

Dynamic Range vs. Qubit®

DeNovix dsDNA Ultra High Sensitivity Assay

Range: 0.5 pg/µL to 300 pg/µL dsDNA
Kit options: 1000 assays, 250 assays or 50 assay evaluation size
Excitation: 470 nm
Emission: 514-567 nm

Dynamic Range vs. Qubit®

Dynamic Range vs. Qubit®