The DeNovix dsDNA Fluorescence Quantification Assays provide the greatest sensitivity and widest dynamic range available today. DeNovix offers three highly specific assay kits for a wide range of dsDNA concentrations and one that covers a wide range RNA concentrations.

DeNovix kits selectively and accurately measure dsDNA even in the presence of common contaminants such as RNA, ssDNA or protein. Combine the specificity of fluorescence with the purity measurements of absorbance on our DS-11 FX range for the optimum sample QC procedure.

Features

  • Widest Dynamic Range: 0.5 pg/µL – 4000 ng/µL
  • 20X Greater Sensitivity than ThermoFisher Qubit™ (Qubit™)
  • Integrated into EasyApps™ software
  • Simple Mix-and-Measure Assay

Assay Choices for Your Application

DeNovix dsDNA assays cover three overlapping ranges of sensitivity. Each kit includes standards for easy set up of a two-point standard curve. DeNovix assays have a wider dynamic range than Qubit™ dsDNA assays – and are more affordable.

Our breakthrough dsDNA Ultra High Sensitivity assay allows measurement of samples as low as 0.5 pg/µL (original sample concentration), 20X lower than the core range of the Qubit™ dsDNA HS assay.

DeNovix dsDNA assays may be used on instruments other than the DeNovix DS-11 FX and QFX series of fluorometers. Use the Detailed Protocol links below for additional information. Click here for downloadable Qubit™ fluorometer .qbt files for use on Qubit™ 2.0 and 3.0 fluorometers.

DeNovix Fluorescence Quantification Assays

DeNovix Fluorescence Quantification Assay Ranges. Image Credit: DeNovix.

Microplates

For those labs requiring higher throughput, all DeNovix assays are also compatible with microplate formats.

Comparison of DeNovix and Thermo Fisher Qubit™ Assay Kits

DeNovix Assays offer an enhanced dynamic range over comparable Thermo Fisher Qubit™ assays, giving researchers greater flexibility and avoiding the need for repeated dilutions. The single DeNovix RNA Assay has 1.5 x the dynamic range of both Thermo Fisher Qubit™ assays combined. 

DeNovix dsDNA Broad Range Assay
Range: 0.1 ng/µL to 2000 ng/µL (extended to 4000 ng/µL) dsDNA
Kit options: 1000 assays, 250 assays or 50 assay evaluation size
Excitation: 375 nm
Emission: 435-485 nm

DeNovix dsDNA High Sensitivity Assay
Range: 5 pg/µL to 250 ng/µL dsDNA
Kit options: 1000 assays, 250 assays or 50 assay evaluation size
Excitation: 470 nm
Emission: 514-567 nm

DeNovix dsDNA Ultra High Sensitivity Assay
Range: 0.5 pg/µL to 300 pg/µL dsDNA
Kit options: 1000 assays, 250 assays or 50 assay evaluation size
Excitation: 470 nm
Emission: 514-567 nm

DeNovix RNA Assay
Range: 250 pg/µL to 1500 ng/µL RNA
Kit options: 1000 assays, 250 assays or 50 assay evaluation size
Excitation: 634 nm 
Emission: 671 nm

Disclaimer: Qubit™ is a registered trademark of Thermo Fisher Scientific.