Discover MiD® Platform—A one box solution for mass spectrometry detection

Microsaic’s patented MiD products and accompanying software deliver quick insights into biological or chemical samples and reactions using miniaturized chip-based technologies.

Microsaic 4500 MiD®

Video Credit: Microsaic Systems plc

The vacuum system, electronics, and computer are all housed inside the 4500 MiD®. It can be installed in places where no other mass spectrometer can be simply deployed, all while maintaining the performance of a normal mass spectrometer system.

With the inclusion of the Microsaic MiDas compact interface sampling module, the 4500 MiD® can now be used in a wide range of on-line, at-line, and off-line applications.

  • Small footprint allows deployment anywhere inside the laboratory or processing facilities
  • Low maintenance costs with a completely tool-free front-end
  • A wholly integrated and sophisticated system, with no external vacuum pumps, and no external PC
  • User-friendly, easy “plug and play” consumables

Chip-based technology

The 4500 MiD® is a reliable, robust, and user-friendly device that features Microsaic’s three main patented chip-based technologies, the ionchip®, spraychip®, and vac-chip, as well as increased mass range, a entirely tool-free front-end for improved control, an inventive and robust user interface, and improved electronics. A change in front-end alignment has also improved system uptime while lowering contaminant deposition downstream.

  • Vac-ch¡p
  • Ionchip®
  • Spraychip®

Mass range

Improving the mass range to 1400 m/z enables new applications such as peptide analysis using Nano LC-MS, which is appropriate for the 4500 MiD®’s exceptionally effective ESI source and a strong propensity for multiple charging.

The Microsaic 4500 MiD® is simple to operate and maintain, and it also consumes less solvent, nitrogen, and power, lowering operating expenses and providing a greener alternative for mass detection.

Table 1. Technical Spec. Source: Microsaic Systems plc

Technical Spec  
Dimensions 55 x 35 x 25 cm (22 x 14 x 10 in.) (including PC, pump and exhaust)
Weight 32 kg (including PC, pump and exhaust)
Ion Source spraychip® – electrospray ionization source
Ionization Modes Positive and negative ESI
Sample Inlet Compatible with standard capillaries and unions e.g., 1/32” tubing
Flow Rate Range 0.3-2000 µL/min
Mass Analyzer ionchip® – quadrupole mass filter
Mass Range 50-1400 m/z
Mass Accuracy ± 0.3 m/z in full scan*
Mass Resolution 0.7 m/z ± 0.1 @ FWHM*
Sensitivity 10pg of reserpine yields a peak in SIM mode with a S/N ratio of 10:1 (RMS)
Interface vac-chip – off-axis microengineered atmospheric pressure interface
Detector Electron multiplier
Dynamic Range 3-4 orders of magnitude
Scan Modes Full scan, SIM (8m/z channels), simultaneous scan/SIM, and timed SIM
Scan Rate m/z 1,500/s (7,500 pts/s)
Pumping System Integrated oil-free pumps (no floor pump needed)
Software Control Masscape® Clarity, PrepCon, Remote Operations Protocol
Connections Ethernet, 5 x USB
2 x digital in (e.g., LC trigger input), 2 x digital out
4 x in (e.g.; UV Trace), 4 x out (e.g., triggering external device)
1 x RS232 (e.g., syringe pump control)


Table 2. Utility requirements. Source: Microsaic Systems plc

Utility requirements  
Power Requirements Power: 250W (operating), 300 W (peak)
Line voltage: 100-240 V AC±10%
Line frequency: 50/60 Hz
Nitrogen Gas Pressure 2-6 bar (29-87 psi)
Purity (≥99,5%)
6 mm OD push fit connection
Pump Exhaust Exhaust connects to an 8 mm OD push fit