LGC Biosearch Technologies offers high-quality and cost-effective MGB Probes. These dual-labeled hydrolysis probes consist of a 5' fluorescent reporter dye and a 3' Eclipse Dark Quencher (EDQ) coupled to the minor groove binder (MGB) moiety.

Minor groove binder (MGB) probes for qPCR

Image Credit: LGC Biosearch Technologies

Enjoy the benefits of high-quality MGB Probes from Biosearch Technologies, including swift turnaround time, competitive pricing, and no minimum order requirement.

Benefits of MGB Probes

  • Short probes with high specificity
  • High signal-to-noise for increased sensitivity
  • Improved SNP detection
  • Trusted and experienced oligo supplier
  • ISO 9001 and ISO 13485 manufacturing options

Short probes for high specificity and signal-to-noise

By non-covalently binding to the minor groove, the 3' MGB moiety enhances the stability of the target-probe duplex. This improved stability allows for a shorter probe design for increased target specificity and enhanced performance.

Shorter probes exhibit superior quenching efficiency since the dye is in close proximity to the quencher. When coupled with a non-fluorescent quencher like EDQ, the short MGB probe achieves a low background and high signal-to-noise ratio.

Improved SNP/mismatch discrimination

Shorter MGB probes exhibit greater destabilization in the presence of a single base mismatch, particularly when the mismatch is within the minor groove binding region. This signifcantly increases the ∆Tm between mutant versus wildtype, enhancing SNP discrimination.

MGB assays designed for maize SNP identification were run on two different Biosearch Technologies low-volume, high-throughput automation platforms, allowing for liquid handling, thermal cycling, and fluorescence detection/analysis. Assays were run on the IntelliQube in 1.6µL reaction volumes in Array Tape (A and B) and the SNPline workflow in 1µL reaction volumes in 1536-well plates (C and D). Both crude-extracted (A, C and D) and sbeadex-purified (B) DNA gave clear clusters, allowing for clear and easy genotype identification. All assays were run with Biosearch Technologies' BHQ Probe Master Mix. Image credit: LGC Biosearch Technologies

Minor groove binder (MGB) probes for qPCR

Trusted supplier of high-quality oligos

LGC's multi-site oligo production processes provide benefits such as surge capacity, risk mitigation, and manufacturing redundancy. Through exceptional vertical integration, their chemical manufacturing laboratories produce most of the crucial raw materials required for oligo synthesis. This ensures a dependable and secure supply chain, along with consistent quality at every stage of development.

Biosearch Technologies produces its own dyes, quenchers, specialized amidites, CPG, and custom synthesis columns making it a trustworthy partner from the beginning of your project through to its commercial scale-up.

 Image Credit: LGC Biosearch Technologies

Order MGB Probe(s) and primers delivered each in a single tube

MGB ValuMix assays for qPCR gene expression offer the convenience of receiving a customized, dual-labeled MGB probe and primer pair in a single tube. The proportions are precisely delivered according to the user's desired probe-to-primer ratio, ranging from 1:1 to 1:4.5, in any one of our standard buffers (TrisHCI, T10E0.1 or T10E1).

MGB ValuMix assays for SNP genotyping include a single tube containing two distinct MGB probes and a primer pair, with precise amounts for optimal performance. These assays are available as a 40x assay mix upon request.

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