Improve assay sensitivity and specificity with Locked Nucleic Acid oligonucleotides (LNA oligos) from LGC Biosearch Technologies. LNAs are RNA bases in which the ribose is “locked” with a methylene bridge, fixing the base in the C3’-endo conformation.

Locked nucleic acid

Locked Nucleic Acid (LNA) probes for qPCR applications
Image Credit: LGC Biosearch Technologies

Benefits of LNA Probes

LNAs enable a shorter probe design, supporting the assay design for:

  • Improved assay sensitivity
    • Higher quenching efficiency (lower background)
    • Greater signal-to-noise ratio
  • Increased hybridization specificity
  • Targeting AT-rich sequences
  • Better mismatch discrimination in SNP genotyping assays

Order dual-labeled LNA Probes

LNAs for commercial use

You can maximize assay performance by harnessing the combined power of LNA technology and best-in-class BHQ quenchers. The Biosearch Technologies team is ready to help you optimize your assay. Provide the manufacturing team with your precise instructions using this Excel template today. 

Additional advantages:

  • Access LNA probes for multiplexing with a full suite of fluorophores.
  • Insert up to seven LNA bases in the probe sequence.
  • Purified via DUAL-HPLC and sequence verified via uHPLC and MS.

Save time and resources with pre-mixed LNA probes and primers

Ordering LNA probe and primer pairs as a ValuMix assay simplifies the assay setup process.

You can supply the specifications, including the probe-to-primer ratio, for gene expression and qPCR assays, and LGC sends you a unique, dual-labeled LNA probe and primer pair in a single tube.

LGC Biosearch Technologies provides a ValuMix assay for SNP genotyping applications that consists of two custom LNA probes and a primer pair delivered in exact quantities.

Order ValuMix for qPCR and gene expression Order ValuMix for SNP genotyping


Customize your LNA oligo with an extensive menu of modifications

Take advantage of LNA-enhanced oligos, for example with your primers for added specificity in SYBR Green Assays. LGC Biosearch Technologies can synthesize custom oligos with internal LNA modifications. Simply add a "+" sign in front of the base to designate the LNA-base position (+A, +T, +C, +G) and insert up to 20 LNA bases.

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