The NeuraLeap module for Ultima 2Pplus Multiphoton Microscopes is the ideal tool for rapidly imaging across a range of depths. NeuraLeap allows for nearly instantaneous switching between optical planes thanks to its extended depth-of-field laser excitation module with an ultrafast DMD chip.
This system can also be used to define multiple excitation planes of various sizes to selectively image larger volumes in a single optical plane. This module offers increased speed and flexibility compared to the Ultima microscope, allowing researchers to image with greater temporal resolution in the brain.
Ultrafast Z-focusing combined with variable depth-of-field selection
NeuraLeap’s DMD allows rapid (20 µs) switching between planes, thereby allowing the collection of several discrete planes across hundreds of microns of depth.
With no added wait time for the Z-device, this module enhances the rate at which samples can be scanned in Z. There is also no additional delay for larger jumps, and planes can be randomly selected for imaging. This, in turn, will give researchers more freedom and flexibility when collecting volumes.
Image with a large, variable depth of field
The NeuraLeap module can be used to establish an elongated illumination spot that will excite fluorophores in planes of up to 150 microns thick with a 16x objective. This means that resonant-rate imaging of volumes is now possible in areas where optical mixing of the signals is not a concern.
As well as increasing frame rates for volumes, this larger illumination plane can also help to prevent the occurrence of signal changes as a result of Z motions in samples, making data collection more reliable.
Select multiple planes to image simultaneously
NeuraLeap produces two discrete imaging spots along its full depth of field, enabling users to choose various planes of interest to image at resonant-rate speeds, separated by hundreds of microns.
Trickier applications, for example, where cells must be imaged across large distances in Z with high temporal precision, are now possible thanks to this dual-plane imaging feature.
Compatible multiphoton imaging systems
Ultima 2Pplus
The NeuraLeap Module is specifically designed to be used with Bruker's flagship all-optical multiphoton system for imaging and optical manipulations.
Image Credit: Bruker Fluorescence Microscopy