Submicron particle imaging with the FlowCam® Nano

The next-generation dynamic, image analysis tool - FlowCam Nano - is capable of measuring and imaging submicron particles in real-time. With its sub-visible particle analysis, FlowCam Nano can detect objects between 300 nm and 2 μm in size — the smallest of which is detected with light microscopy.

Users can utilize FlowCam Nano for the early detection and monitoring of aggregates and contaminants in protein formulations, nanodrug delivery systems, bacterial characterization, material characterization and bioprocess monitoring.

Submicron particle imaging with the FlowCam® Nano

Image Credit: Yokogawa Fluid Imaging Technologies, Inc.

Use FlowCam Nano for

  • Formulation development
  • Bioprocessing
  • Biomedical research
  • Bacterial contamination detection
  • Gene therapy aggregate monitoring
  • Materials characterization

FlowCam Nano benefits

  • High-quality images to identify and detect submicron particle types for product development and quality control
  • Instrument setup and data collection are quick and easy, thanks to the user-friendly interface and the autofocus technology
  • Rapid detection of API aggregation as well as other degradation modes that may cause large subvisible and visible particles
  • Determine the size and morphology of particles in the 300 nm to 2 µm size range that cannot be detected by traditional flow imaging microscopy
  • VisualSpreadsheet® software that can visualize, classify and characterize nanoparticles in real-time

FlowCam Nano captures submicron images of protein aggregates, silicone oil droplets, sucrose aggregates, and E. coli cells. The particle diameter is shown in µm.

FlowCam Nano captures submicron images of protein aggregates, silicone oil droplets, sucrose aggregates, and E. coli cells. The particle diameter is shown in µm. Image Credit: Yokogawa Fluid Imaging Technologies, Inc.

How it works

Similar to flow imaging microscopy (FIM) instruments, FlowCam Nano consists of a light microscopy apparatus (a stroboscopic LED light source, an objective lens and a high-speed camera) with a flow cell placed in the path of the light source and objective lens.

Analyzing particles is carried out by injecting a sample with a syringe pump. Images of particles passing through the optics are captured and recorded automatically. After analyzing the microscopic images, the size, morphology, concentration and types of particles in the sample can be determined.

Submicron particle imaging with the FlowCam® Nano

Image Credit: Yokogawa Fluid Imaging Technologies, Inc.

A high-magnification (40X) objective lens and a thin layer of immersion oil reduce the numerical aperture between the flow cell, the objective and the condenser of FlowCam Nano, which provides higher resolution than standard flow imaging microscopy.

The patented FlowCam Nanotechnology magnifies particles near and below the size limit of conventional FIM instruments, extending the range of particle detection and imaging capability to submicron particles.

FlowCam Nano specifications

  • Size range of particle: 300 nm to 2 µm
  • Camera: High Resolution (1440 × 1080 pixels) CMOS sensor, monochrome
  • Magnification: 40X
  • Frame rate: up to 130 frames per second
  • Minimum sample volume: 50 µL
  • Flow cell: 60 µm depth
  • Sample processing capability: 20 µL/minute
  • VisualSpreadsheet 5 Software

Explore FlowCam applications

  • Biopharma and pharma
  • Water quality monitoring
  • Plankton research
  • Harmful algal blooms
  • Food and beverage
  • Industry