The InverTau™ platform is a laser scanning confocal unit designed for Fluorescence Lifetime Imaging (FLIM). Fully software-controlled, it mounts seamlessly onto the side port of an inverted fluorescence microscope.
Whether you're building a custom system around InverTau or purchasing a complete setup from HORIBA, the platform offers flexibility to suit your research needs. Plus, versatile configurations come backed by responsive local support.
Features
- Add InverTau to the side port of an inverted microscope or buy a complete solution from HORIBA
- Affordable laser scanning unit for FLIM studies
- Fully computer-controlled confocal optics with the intuitive EzTime Image Software minimizes setup time and increases productivity
- Real time video display up to 6 fps with InverTau and up to 30 fps with addition of FLIMera
- Simply switch from confocal laser scanning to widefield imaging with the addition of FLIMera
InverTau
Video Credit: HORIBA
What’s included
- Computer, electronics and EzTime Image software
- DeltaDiode-C1 controller, CND and fiber coupler for HORIBA DeltaDiode TCSPC sources (optional)
- FLIMera widefield video rate FLIM camera for real time visualization and lifetime kinetics
- HORIBA DeltaDiode lasers from 375 to 595 nm (optional) are perfectly matched for use with this system
- HPPD-720 TCSPC detector (additional models available)
- InverTau confocal fluorescence lifetime imaging platform
- Nikon Ti2-U manual inverted fluorescence microscope
Image Credit: HORIBA
EzTime Image software
EzTime Image is an intuitive, touchscreen-optimized software designed for use with HORIBA’s InverTau, Flimera, and stage scanning systems. It offers fully automated control for TCSPC and MCS acquisition and analysis, making it easy to manage complex workflows.
As a complete application, EzTime Image handles:
- Data analysis
- Instrument recognition and control
- Data collection
Image Credit: HORIBA
Specifications
InverTau Specifications. Source: HORIBA
| . |
. |
| Modes |
Single point, raster, bi-directional (xy) |
| Scan speed |
Up to 6 fps (bi-directional) at 512 × 512 |
| Image resolution |
32 × 32 to 4k × 4k |
| Digital zoom |
1 FoV to 0.1 FoV |
| Attachment |
To side port of inverted microscope (C-mount) |
| Control software |
EzTime™ Image software |
| Optical ports |
2 on excitation, 2 on emission |
| Attenuation filters |
Steps from 0-6 OD ND |
| Dichroic filters |
400 nm, 450 nm, 500 nm, 550 nm, 600 nm, 730 SP, 50/50 BS |
| Emission filters |
400 BP, 450 BP, 500 BP, 550 BP, 600 BP, 440 LP, 475 LP, 530 LP, 570 LP, 630 LP |
| Pinhole |
10, 25, 50, 100, 200 μm (+ closed and fully open) |
| Photon streaming |
Stream to HDF5 |
| Phasor analysis |
Switchable with histogram |
| Data analysis |
Global up to 5 exponentials |
Key FiPho Electronics Specifications for FLIM. Source: HORIBA
| . |
. |
| Lifetimes from |
<50 ps |
| Deadtime |
5 ns |
| TCSPC bin width |
<15 ps |
| Histogram Bin Depth |
8, 16 and 32-bit |
| Independent Stops |
1 to 4 |
| Maximum Start Rate |
100 MHz |
| Maximum Rate/Stop Channel |
10 Mcps |
| Operating Mode |
Automatic Forward Timing |
| Streaming Mode |
Photon Streaming (Time-Tag) |
| Acquisition and Analysis Macro Scripting |
Yes |
| PC Interface |
USB 3.0> |
| Software |
EzTime™ Image |