OT-2, for pipetting automation needs

A bench-top liquid handler with enough flexibility to automate numerous common applications is the OT-2.

Video Credit: Opentrons

Automate the most time-consuming applications in the lab

General liquid handling

The easiest method for making time in the lab. The OT-2 pipetting robot makes it simple to automate various fundamental liquid handling operations, such as sample pooling, cherry picking, serial dilution, tube and microplate filling, and normalization. Users can design their own protocol, ask the Applications Engineering team to create one for them or select from various verified protocols in the Protocol Library.

Image Credit: Opentrons 

PCR setup

Another common use for automation particularly when preparing a large number of samples. To mix samples and PCR mastermix in the reaction plate that will be placed in the thermocycler or qPCR machine, use the OT-2 pipetting robot.

Image Credit: Opentrons 

Protein purification

The most economical method for processing proteomics samples, enzymatic and chemical lysis, and small-scale protein purification on an automated basis. Two automation approaches are available for the OT-2 pipetting robot: dual-flow chromatography-based automation and magnetic bead-based automation.

Image Credit: Opentrons 

Small footprint, big impact

Tech specs

Source: Opentrons

Dimensions	63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H) Takes up half of your standard lab bench
Weight	48 kg or 105.8 lb
Pipette configurations	Single- and 8-channel pipetting 2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.
Pipette volumes and specs	Single-channel: 1-1000 µL 8-channel: 1-300 µL
Deck	11 deck slots enable countless configurations Deck slots are compatible with standard SBS dimensions. Deck also includes a removable trash bin.
Connectivity	WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
Video	Integrated video camera (Included for future or custom functionality)
Audio	Integrated audio system (Included for future or custom functionality)
Power requirements	Robot Power Input: 36 VDC, 6.1 A Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC For additional power requirement information, please review this support article
Frame composition	Rigid steel and CNC aluminum design
Operating temperature & relative humidity	Recommended 20-24 °C Recommended 40-60% RH
Time to fill 96-well plate	Pipette Distribute, one tip Transfer, same tip Transfer, new tip P20 8-Channel 0:22 1:35 4:19 P300 8-Channel 0:26 1:38 4:24 P20 Single-Channel 3:12 13:10 36:01 P300 Single-Channel 4:01 13:39 41:31 P1000 5:54 15:21 44:50
Single-channel accuracy & precision	Pipette Volume Random Error Systematic Error     %D µl %CV µl P20 1 10 20 ±15% ±2% ±1.5% 0.15 µl 0.2 µl 0.3 µl ±5% ±1% ±0.8%   P300 20 150 300 ±4% ±1% ±0.6% 0.8 µl 1.5 µl 1.8 µl ±2.5% ±0.4% ±0.3%   P1000 100 500 1000 ±2% ±1% ±0.7% 2.0 µl 5.0 µl 7. 0µl ±1% ±0.2% ±0.15%
8-channel accuracy & precision	Pipette Volume Random Error Systematic Error     %D µl %CV µl P20 1 10 20 ±20% ±3% ±2.2% 0.2 µl 0.3 µl 0.44 µl ±10% ±2% ±1.5%   P300 20 150 300 ±10% ±2.5% ±1.5% 2.0 µl 3.75 µl 4.5 µl ±4% ±0.8% ±0.5%
Sterility	Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment Add the Opentrons HEPA Module, or add your own UV lights
Viscosity	For best practices on automating viscous liquid handling, review our application note Viscous Liquid Handling Automation using Opentrons OT-2 ›
API compatibility	Interfaces with any product that has an API Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
Interface	Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later. Opentrons App
Tip compatibility	Use Opentrons Tips and Opentrons Filter Tips Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
Labware compatibility	Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
Warranty	One-year warranty against manufacturer defects Stress tested to last a minimum of 3 years of full-time use
Return policy	60 days, 100% risk-free
Certifications	Certifications complete (CE, FCC, NRTL, CB, ISO 9001) Not certified/validated (IVD, GMP)
Example Commands	Tip Handling Pick up tip Drop tip Return tip New tip (always, never, once) More… Liquid Control Aspirate Dispense Aspirate speed Dispense speed Blowout Touch tip Mix Air gap Utility Move to Delay User-specified pause Homing Comment Transfer Basic transfer Volumes > Pipette Volume Distribute Consolidate Variable Settings Speed Volume Count Other Dispose
What’s in the box	OT-2 robot Calibration block Removable block Trash container Quickstart guide Power supply and regional IEC cable Ethernet cable Ethernet-to-USB dongle Tool kit

Use what is provided, add what is needed

Image Credit: Opentrons 

Source: Opentrons 

Choose the protocol method that is right for the user

The code that directs the robot through the experiment is called a protocol. The company has developed several methods for developing a protocol that anyone can use, regardless of experience level with Python coding or automation.

Custom Protocol

Ideal for users desiring a fast, unique, or complex protocol. Has a protocol designed specifically for experiments by experts.

Protocol library

Ideal for novices wishing to acquire experience. Examine open-source, well-documented protocols.

Protocol designer

Ideal for people who wish to develop a new protocol without knowing how to code. Create new protocols with a user-friendly drag-and-drop interface.

Python protocol API

Ideal for users who have knowledge of Python. They can use the open-source Python Protocol API to create their own protocol.

Space for two pipettes

Dependable liquid transfers between 1 and 1000 µL using interchangeable single- and 8-channel pipettes

Image Credit: Opentrons

SINGLE-CHANNEL

• P20
• P300
• P1000

Image Credit: Opentrons

 8-CHANNEL

• P20
• P300

Image Credit: Opentrons