SCIEX latest accurate mass spectrometry solution: The ZenoTOF 7600 system

The ZenoTOF 7600 system is an accurate mass spectrometry solution that combines powerful MS/MS sensitivity, innovative fragmentation technology and a step-change in data independent acquisition (DIA). Unlock sensitivity gains that reveal new, rare or even previously undetected information every day.

A powerful leap in proprietary innovation, this accurate mass system combines the power of the Zeno trap with electron activated dissociation (EAD) fragmentation. Detect up to 20x more ions in every experiment and access a spectrum of tunable fragmentation techniques to uncover new perspectives for every molecule, in every experiment. And now, Zeno SWATH DIA capitalizes on these gains, revealing tens, hundreds and even thousands more identified and quantified analytes in a shorter time, and with higher precision than ever before. Reaching new depths of coverage, Zeno SWATH DIA delivers maximal information in minimal time.

The ZenoTOF 7600 system enables you to:

  • Characterize large molecules, including post-translational modifications (PTMs)
  • Elucidate positional isomers on small molecules and lipids
  • Identify and quantify proteins and peptides at unparalleled speed
  • Deliver a high depth of coverage, particularly on low abundance species, quickly and robustly

The Zeno revolution is now

  • Overcome QTOF MS/MS duty cycle deficiencies, with >90% ions injected into the TOF
  • Achieve sensitivity gains of 50–20x with Zeno trap
  • Identify and quantify low abundance species
  • Enable tunable fragmentation of all molecule types
  • Utilize controlled EAD
  • Reach MS/MS scan rates of up to 133 Hz
  • Improved DDA, DIA and MRMHR

Zeno trap

The next era of sensitivity for accurate mass

Ions are accumulated in the Zeno trap before being pulsed rapidly into the TOF, meaning up to 20x more fragment ions can be detected. Enabled by a new level of sensitivity, each MS/MS experiment contains more useful information than ever before - particularly on lower abundance species that were previously undetectable.

Zenotof 7600 System for high-resolution mass spectrometry

Image Credit: SCIEX 

Zeno SWATH DIA enabled by the Zeno trap

Zeno SWATH DIA combines the power of the Zeno trap with SWATH DIA. Similar to SWATH DIA, Zeno SWATH DIA identifies and quantifies analytes using MS/MS data. Thus, the rapid acquisition of high-quality MS/MS data across the entire precursor ion space is fundamental to operation.
In Zeno SWATH DIA, the Zeno trap, when activated, is used to increase the MS/MS sensitivity for each variable window acquired. The Zeno trap provides a 4-20x gain in sensitivity for Zeno SWATH DIA, while also maintaining other key performance attributes.

Electron Activated Dissociation (EAD)

A step change in fragmentation technology: using EAD when CID is not enough

The ability to tune electron kinetic energy extends the utility of the approach to all molecule types, from singly charged small molecules to large multiply charged proteins. EAD allows for a range of reagent-free electron-based fragmentation mechanisms with a single device, and it has the capability to fragment peptides while retaining critical MS/MS information for both identification and localization of PTMs. Unlike other electron based fragmentation techniques, EAD delivers reproducible, consistent data, even at fast scan speeds, and it is compatible with UHPLC time frames, delivering higher efficiency than electron-transfer dissociation (ETD). In this instrument, geometry coupling EAD with the Zeno trap allows for detection of very low abundant diagnostic fragment ion species, leading to greater sequence coverage.

Zeno MRMHR unlocks new levels of sensitivity

Zeno trap pulsing on demand offers the capacity to sense lower abundance ions simultaneously as those in abundance, which redefines the quantification limits that can be attained with accurate mass. MRMHR is a targeted approach, providing high selectivity and sensitivity for targeted and screening quantification.

Zenotof 7600 System for high-resolution mass spectrometry

Targeted peptide quantification - Significant sensitivity gains in MS/MS. Comparison of extraction ion chromatograms (XICs) for LILTLTHGTAVC[CAM]TR fragments obtained from MS/MS collected with Zeno trap on vs. Zeno trap off. Signal/noise ratio improved ~8 fold when using the Zeno trap. Image Credit: SCIEX

If you would like more information about how to use the ZenoTOF 7600 system for your application, do not hesitate to contact us and/or download the brochure below with examples of the system in action.