Using the iQue® SARS-CoV-2 (IgG, IgM and IgA) Kit, users are able to explore COVID-19 research and obtain a comprehensive profile for SARS-CoV-2 using a multiplex and high-throughput serological assay. This kit from Sartorius allows for the qualitative and quantitative analysis of anti-SARS-CoV-2 Spike RBD antibodies from human blood samples (sera and plasma) using a simple workflow that is designed with user-friendliness in mind.
Image Credit: Sartorius
Top features of the iQue® SARS-CoV-2 (IgG, IgM and IgA) kit
- Integrated detection of all three antibody isotypes helps save time and valuable samples
- Precise immune response assessment
- High throughput and simplified analysis can be performed for SARS-CoV-2 serological surveillance
Popular applications for the iQue® SARS-CoV-2 (IgG, IgM and IgA) kit
- Vaccine development
- Virology research
The outbreak of SARS-CoV-2 continues to persist as a result of its ability to constantly mutate and evade immunization. Protection against the virus is thus dependent on the development of a humoral immune response, which means that there is still an ongoing need for the accurate assessment of quantity and duration of antibody response (particularly those targeting the SARS-CoV-2 spike protein receptor binding domain).
To facilitate characterization and monitor the duration of the antibody response in both COVID-19 patients and vaccinated individuals, detect asymptomatic infections and survey past infection prevalence in a population, serological testing to quantitate the three major isotypes (IgG, IgM and IgA) of antibodies specific for SARS-CoV-2 Spike RBD can be used.
With the help of the iQue® SARS-CoV-2 (IgM, IgG, and IgA) Kit, detection is quick and trustworthy.
Innovation for smarter research
While it is possible to identify anti-SARS-CoV-2 Spike RBD IgG, IgM, or IgA antibodies using conventional enzyme-linked immunosorbent assay (ELISA), flow cytometry, and lateral flow immunoassay, these techniques have drawbacks:
- Provide only qualitative results
- Labor-intensive and do not support applications with high throughput
- Requires massive amounts of reagents
- IgG, IgM, and IgA antibody multiplexing analysis is not permitted
The iQue® SARS-CoV-2 (IgG, IgM, and IgA) kit offers a quick, strong, and sensitive serological assay for measuring IgM, IgG, and IgA anti-SARS-CoV-2 Spike RBD antibodies and is available in a single well of a 96- or 384-well plate along with antibody detection levels in the pg/mL range.
The multiplexed assay allows for the high-throughput analysis of the three major isotypes (IgG, IgM and IgA) of antibodies specific for SARS-CoV-2 Spike RBD in a single well for a comprehensive analysis of the immune response.
Save precious samples
Users can preserve laboratory samples by using a streamlined workflow that only requires a single 10 µL sample diluted at a minimum of 100-fold. Sartorius' kit enables users to obtain reliable and accurate data without exhausting valuable resources like reagents.