Small footprint. Low budget. Large impact. With icon16, laboratories using smaller sequencing batches may use the entire AutoNorm standard without sacrificing data quality or having to wait to fill a plate. Perfect for smaller sequencing facilities, translational research teams, clinical research initiatives, and PI laboratories.
icon16™
Compact footprint. Cost-effective operation. Significant impact.
Image Credit: n6 Tec, Inc.
Featuring AutoNorm™ technology for adaptive amplification cycle control.
Image Credit: n6 Tec, Inc.
From chaos to order
The world’s first real-time thermocyclers with individually controlled wells
Individual miniaturized thermocyclers with real-time temperature monitoring. Image Credit: n6 Tec, Inc.
First, N6 Tec created a better box in a 16- or 96-well configuration, bringing the newest technologies from the telecom and semiconductor industries to the field of genomics.
Adaptive amplification
AutoNorm technology seamlessly normalizes a 2048-fold input range across these 36 samples, delivering perfectly uniform library yields without a single over-amplified well. Image Credit: n6 Tec, Inc.
Then, they designed it to be smart; AutoNorm technology tracks and prevents cycling in line with user-specified parameters.
RNA input
Image Credit: n6 Tec, Inc.
Amplification curves
Image Credit: n6 Tec, Inc.
The above graphs show data generated by the HudsonAlpha Institute. icon96 was used to amplify plant-derived RNA samples that ranged in size from 0.5 to 1.5 micrograms. AutoNorm was used to dynamically choose each sample's stop cycle depending on the amplification curve's slope. As a result, read alignment improved, normalization was simplified, and adapter dimer in the libraries was reduced to less than 0.02%.
icon16™ offers even more
Universal interoperability
Open chemistry, sequencing platform agnostic
Simplified workflow
No requirement for individual normalization or quantification
Automation ready
Complete API package, standard plates and strips
Cost-saving
Reduces labor and consumable costs
Cleaner, better data
Minimizes dropout and artifacts
Compliant
ISO 13485-compliant
icon technology can be used for a variety of sequencing applications
- Single cell
- Spatial
- Whole genomes
- RNAseq
- Assay development
- Degraded/FFPE-extracted
- 16s metagenomics
- cfDNA/liquid biopsy
- Genotyping