Understanding the histone code through single-cell analysis

Recent epigenetic discoveries have highlighted the complexity of the histone code, yet most measurements still rely on bulk analyses, which mask cell-to-cell variation. This prompts the question: what if the most significant regulatory signals are lost in the averaging?

In a recent webinar, Ronald Cutler, PhD, described a mass-spectrometry-based approach to characterizing histone post-translational modifications at true single-cell resolution using the cellenONE platform.

His team at Albert Einstein College of Medicine in New York directly quantified dozens of histone modification states, revealing remarkable chromatin variability in ostensibly homogenous cell populations.

This webinar explored a variety of topics:

• Why researching chromatin states at the single-cell level is important in epigenetic studies
• How the cellenONE platform facilitates high-throughput sample preparation for omics research
• How single-cell profiling uncovers differential responses to epigenetic perturbations, revealing subpopulations and coordinated modification patterns not seen in bulk data
• How this technique provides unprecedented resolution for understanding epigenetic regulation, biological states, and treatment responses

About the speaker

Ronald Cutler, Ph.D., is a Postdoctoral Researcher at Albert Einstein College of Medicine in New York. His work focuses on advancing single-cell epigenetic analysis, with particular expertise in mass spectrometry-based profiling of histone post-translational modifications.