Utilize the in vitro 3D Non-alcoholic steatohepatitis (NASH), also known as Metabolic-dysfunction-associated steatohepatitis (MASH) model to expedite the path to effective treatment development.

Everything needed to replicate the industry-validated human in vitro NASH model in the user’s own laboratory is included in NASH-in-a-box.

It is intended to be used with the single- and multi-organ microphysiological systems in the PhysioMimix OOC range.

NASH-in-a-box supports:

  • The swift acceptance of an organ-on-a-chip methodology by scientists, regardless of their background
  • Instant access to protocols that have been verified and improved over years of industry cooperation
  • Producing clinically translatable data with high content to reveal disease mechanisms and validate medication safety and effectiveness

Features of the 3D NASH model kit

Use NASH-in-a-box to overcome the drawbacks of conventional methods and prevent unanticipated outcomes in the clinic.

  • 10-month shelf life (from manufacture)
  • ELISA quality control kits (albumin/cytotoxicity) to test microtissue health and function
  • PhysioMimix OOC compatible and fully supported
  • Guaranteed  for 14 days of culture
  • Proprietary NASH media and supplements
  • Software-guided protocols
  • 3D-validated primary human hepatocytes, stellate, and Kupffer cells

What is in the box?

Media. Source: CN-Bio

Media Volume
Hepatocyte thawing media 50 mL
Basal media (HEP-Fat) 500 mL
Basal media (HEP-Stellate) 250 mL
Basal media (HEP-Seeding) 250 mL


Supplements. Source: CN-Bio

Supplements Volume
HEP- Supplement A 5 x 2.5 mL
HEP Supplement B 1 x 250 µL  
HEP- Supplement C 1 x 500 µL  
HEP- Supplement D 1 x 500 µL  
Hydrocortisone 5 x 5 µL  
TGF – Beta 5 x 200 µL 


Assay kits. Source: CN-Bio

Quality control assays Number of kits
Assaypro Human Albumin AssayMax ELISA Kit 2 kits
Promega CytoTox 96 Cytotoxicity (LDH) Assay Kit 1 kit


Primary human cells. Source: CN-Bio

Primary human cells Number of cells  
Hepatocytes min 10M
Kupffer cells min 1M
Stellate cells min 100K


PhysioMimix plates. Source: CN-Bio

Multi-chip plates   Number of plates/replicates
Multi-chip Liver-12+ plate (MPS-LC12+) 2 plates /24 replicates  


Validation report

The lengthy procedure of verifying donor cell combinations within NASH-in-a-box is detailed in the sample report.

When cultured for 14 days, the quality control protocols guarantee the formation of stable, three-dimensional liver microtissues that exhibit a NASH phenotype.

PhysioMimix® Compatible NASH-in-a-Box

Image Credit: CN-Bio

Guided software

Guided software, which can be found on the PhysioMimix Controller, offers detailed instructions for culturing liver microtissues and causing the NASH phenotype.

PhysioMimix® Compatible NASH-in-a-Box

Image Credit: CN-Bio

It helps operators with little experience to confidently use the NASH-in-a-box kit by walking them through the process.

How does the 3D NASH model compare?

Preclinical research and development instruments are widely accessible. How does NASH-in-a-box compare to established approaches?

Source: CN-Bio

  In vitro 2D
cell culture
In vitro 3D
In vivo 
animal models
Human relevance ++ ++ + +++
Longevity < 7 days < 7 days > 4 weeks 2 weeks
Model complexity + + +++ ++
Throughput +++ +++ + ++
Ease of adoption +++ ++ + ++
Cost $ $ $$$ $$
NASH phenotype + ++ + +++

Related Consumables

Other Equipment by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.