Utilize the in vitro 3D Non-alcoholic steatohepatitis (NASH), also known as Metabolic-dysfunction-associated steatohepatitis (MASH) model to expedite the path to effective treatment development.
Everything needed to replicate the industry-validated human in vitro NASH model in the user’s own laboratory is included in NASH-in-a-box.
It is intended to be used with the single- and multi-organ microphysiological systems in the PhysioMimix OOC range.
NASH-in-a-box supports:
- The swift acceptance of an organ-on-a-chip methodology by scientists, regardless of their background
- Instant access to protocols that have been verified and improved over years of industry cooperation
- Producing clinically translatable data with high content to reveal disease mechanisms and validate medication safety and effectiveness
Features of the 3D NASH model kit
Use NASH-in-a-box to overcome the drawbacks of conventional methods and prevent unanticipated outcomes in the clinic.
- 10-month shelf life (from manufacture)
- ELISA quality control kits (albumin/cytotoxicity) to test microtissue health and function
- PhysioMimix OOC compatible and fully supported
- Guaranteed for 14 days of culture
- Proprietary NASH media and supplements
- Software-guided protocols
- 3D-validated primary human hepatocytes, stellate, and Kupffer cells
What is in the box?
Media. Source: CN-Bio
| Media |
Volume |
| Hepatocyte thawing media |
50 mL |
| Basal media (HEP-Fat) |
500 mL |
| Basal media (HEP-Stellate) |
250 mL |
| Basal media (HEP-Seeding) |
250 mL |
Supplements. Source: CN-Bio
| Supplements |
Volume |
| HEP- Supplement A |
5 x 2.5 mL |
| HEP Supplement B |
1 x 250 µL |
| HEP- Supplement C |
1 x 500 µL |
| HEP- Supplement D |
1 x 500 µL |
| Hydrocortisone |
5 x 5 µL |
| TGF – Beta |
5 x 200 µL |
Assay kits. Source: CN-Bio
| Quality control assays |
Number of kits |
| Assaypro Human Albumin AssayMax ELISA Kit |
2 kits |
| Promega CytoTox 96 Cytotoxicity (LDH) Assay Kit |
1 kit |
Primary human cells. Source: CN-Bio
| Primary human cells |
Number of cells |
| Hepatocytes |
min 10M |
| Kupffer cells |
min 1M |
| Stellate cells |
min 100K |
PhysioMimix plates. Source: CN-Bio
| Multi-chip plates |
Number of plates/replicates |
| Multi-chip Liver-12+ plate (MPS-LC12+) |
2 plates /24 replicates |
Validation report
The lengthy procedure of verifying donor cell combinations within NASH-in-a-box is detailed in the sample report.
When cultured for 14 days, the quality control protocols guarantee the formation of stable, three-dimensional liver microtissues that exhibit a NASH phenotype.
Image Credit: CN-Bio
Guided software
Guided software, which can be found on the PhysioMimix Controller, offers detailed instructions for culturing liver microtissues and causing the NASH phenotype.
Image Credit: CN-Bio
It helps operators with little experience to confidently use the NASH-in-a-box kit by walking them through the process.
How does the 3D NASH model compare?
Preclinical research and development instruments are widely accessible. How does NASH-in-a-box compare to established approaches?
Source: CN-Bio
| |
In vitro 2D
cell culture |
In vitro 3D
spheroid |
In vivo
animal models |
NASH-in-a-
box |
| Human relevance |
++ |
++ |
+ |
+++ |
| Data content |
LOW |
MEDIUM |
HIGH |
HIGH |
| Longevity |
< 7 days |
< 7 days |
> 4 weeks |
2 weeks |
| Model complexity |
+ |
+ |
+++ |
++ |
| Throughput |
+++ |
+++ |
+ |
++ |
| Ease of adoption |
+++ |
++ |
+ |
++ |
| Cost |
$ |
$ |
$$$ |
$$ |
| Time to result |
DAYS |
DAYS |
MONTHS |
WEEKS |
| NASH phenotype |
+ |
++ |
+ |
+++ |