Bio-Synthesis Urine Exosome RNA Isolation Kit constitutes an all-in-one system for the concentration and isolation of exosomal RNA from urine and tissue culture media.
Purification is based on spin column chromatography using Bio-Synthesis proprietary resin as the separation matrix. Briefly, the urine samples are first centrifuged in order to remove all cells.
The urine exosomes are then separated from the urine using Bio-Synthesis proprietary resin. Next, the exosomes are lysed to release the RNA, which is then bound to Bio-Synthesis resin (BIND). Under these conditions only the RNA will bind to Bio-Synthesis resin while the remaining contaminants are removed in the flowthrough or retained on top of the resin.
The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified exosome RNA is eluted into 100 µL of the provided Elution Buffer (ELUTE). Refer the procedure flowchart given below.
The exosomal RNA is isolated free from inhibitors and can be used as template in a number of sensitive downstream assays. The kit is designed to allow for the isolation of exosomal RNA from 50 urine samples. The kit is designed to allow for the isolation of exosomal RNA from 1 mL to 10 mL urine samples.
Features and Benefits
- Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in under 30 minutes.
- Versatile urine input volume - Isolate urine exosomal RNA from 1 mL to 10 mL of urine samples.
- No phenol:chloroform extractions - Bio-Synthesis Urine Exosome RNA Isolation Kit isolates RNA without the use of harmful chemicals such as phenol or chloroform.
- Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA is fully compatible with various downstream applications relating to gene regulation and functional analysis, including RT-PCR, northern blotting and microarray analysis.
The purified urine exosome RNA is compatible with most of the molecular biological applications such as:
- Quantitative, real-time RT-PCR for
- Northern blotting
- RNase protection
- Primer extension
- Expression array assays
- Next Generation Sequencing
- RNA Cloning